Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture
| Autor: | Adriana Trapani, María Ángeles Esteban, Francesca Curci, Daniela Erminia Manno, Antonio Serra, Giuseppe Fracchiolla, Cristóbal Espinosa-Ruiz, Stefano Castellani, Massimo Conese |
|---|---|
| Přispěvatelé: | Trapani, Adriana, Ángeles Esteban, María, Curci, Francesca, Manno, Daniela Erminia, Serra, Antonio, Fracchiolla, Giuseppe, Espinosa-Ruiz, Cristóbal, Castellani, Stefano, Conese, Massimo |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Fish Proteins
Polyphenol solid lipid nanoparticles grape seed–derived extract drug delivery X-ray diffraction antioxidant activity fish cells Pharmaceutical Science Organic chemistry solid lipid nanoparticle Aquaculture Article Antioxidants Analytical Chemistry QD241-441 Nanoparticle Drug Discovery Animals Vitis Physical and Theoretical Chemistry Grape Seed Extract Animal Fishes Polyphenols fish cell Oxidative Stre Liposome Oxidative Stress Gene Expression Regulation Chemistry (miscellaneous) Fish Protein Liposomes Molecular Medicine Nanoparticles Antioxidant Fishe |
| Zdroj: | Molecules Molecules, Vol 27, Iss 344, p 344 (2022) Molecules; Volume 27; Issue 2; Pages: 344 |
| Popis: | The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire® 50/13 and Precirol® ATO5 to administer a grape seed extract (GSE) mixture containing several antioxidant compounds. The combination of the two lipids for the SLN formation resulted in colloids exhibiting mean particle sizes in the range 139–283 nm and zeta potential values in the range +25.6–43.4 mV. Raman spectra and X-ray diffraction evidenced structural differences between the free GSE and GSE-loaded SLN, leading to the conclusion that GSE alters the structure of the lipid nanocarriers. From a biological viewpoint, cell lines from gilthead seabream and European sea bass were exposed to different concentrations of GSE-SLN for 24 h. In general, at appropriate concentrations, GSE-SLN increased the viability of the fish cells. Furthermore, regarding the gene expression in those cells, the expression of antioxidant genes was upregulated, whereas the expression of hsp70 and other genes related to the cytoskeleton was downregulated. Hence, an SLN formulation containing Gelucire® 50/13/Precirol® ATO5 and GSE may represent a compelling platform for improving the viability and antioxidant properties of fish cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|