Influence of the indigenous gastrointestinal microbial flora on duodenal Mg2+ -dependent and (Na+ + K+) -stimulated adenosine triphosphatase activities in mice
| Autor: | D P Yolton, D C Savage |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Cations
Divalent Duodenum ATPase Sodium Immunology chemistry.chemical_element Biology Microbiology Ouabain Divalent Jejunum Mice Cecum medicine Animals Magnesium Adenosine Triphosphatases chemistry.chemical_classification Mice Inbred ICR Stomach Cations Monovalent Molecular biology Specific Pathogen-Free Organisms Infectious Diseases medicine.anatomical_structure chemistry Biochemistry Potassium biology.protein Parasitology Research Article medicine.drug |
| Zdroj: | Infection and Immunity. 13:1193-1198 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.13.4.1193-1198.1976 |
| Popis: | Mg-dependent and (Na+ + K+)-stimulated adenosine triphosphatase (ATP-ase) activities were assayed in butanol extracts of duodenal tissue from germ-free, specific-pathogen-free, and ex-germfree mice associated with an indigenous microflora from specific-pathogen-free mice. In the germfree mice the levels of both ATPase activities were significantly higher than the levels in specific-pathogen-free mice. By contrast, the ex-germfree animals colonized by an entire indigenous microflora, the values fell to levels close to those for specific-pathogen-free animals. (Na+ + K+)-stimulated ATPase was not inhibited by ouabain in extracts from any of the three kinds of mice. These findings show that the indigenous microbial flora influences the intestinal ATPase activity of mice and, because of the connection between (Na+ + K+-stimulate ATPase and active transpoort, undoubtedly affects the process of absorption in the intestinal tract. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|