Evaluation of CCR3 as a basophil activation marker
| Autor: | Gregory J. Philipps, Aaruni Khanolkar, Benjamin J Hansen, Steven J. Burden, Harry R. Hill, Andrew Wilson |
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| Rok vydání: | 2013 |
| Předmět: |
Receptors
CCR3 CCR3 Down-Regulation chemical and pharmacologic phenomena Receptors Fc Basophil Immunoglobulin E Histamine Release Sensitivity and Specificity Flow cytometry chemistry.chemical_compound immune system diseases parasitic diseases medicine Humans Receptor medicine.diagnostic_test biology Receptors IgE hemic and immune systems General Medicine Basophils Basophil activation medicine.anatomical_structure chemistry Immunology biology.protein Antibody Histamine Biomarkers |
| Zdroj: | American journal of clinical pathology. 140(3) |
| ISSN: | 1943-7722 |
| Popis: | Objectives: Recent reports have provided conflicting evidence on the stability of CCR3 expression on the surface of basophils. Hence we wanted to independently evaluate the diagnostic usefulness of CCR3 as a surrogate marker of basophil activation and function. Methods: We examined the correlative relationship between CCR3 expression on the surface of donor basophils and histamine release after donor basophils were treated with agonistic antibodies directed against the high-affinity IgE-Fc receptor and serum samples from 80 individuals displaying symptoms of chronic urticaria (CU). Results: We observed that CCR3 was significantly downregulated on donor basophils treated with the agonistic antibody and CU-patient serum that demonstrated positive “histamine-releasing activity” (HRA scores >10). However, CCR3 downregulation was also observed on donor basophils incubated with more than 40% of CU-patient serum samples with HRA scores less than or equal to 10. Conclusions: Overall our data show that CCR3 demonstrates adequate sensitivity (83%) but weak specificity (59%) in its ability to reliably identify histamine-releasing activated basophils. |
| Databáze: | OpenAIRE |
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