Purification and Characterization of a Black Walnut (Juglans nigra) Allergen, Jug n 4
| Autor: | Andrew L. Thomas, Tara H. McHugh, Jiang Yi, Yuzhu Zhang, Yuting Fan, Wen-Xian Du, Kari C. Nadeau, Shu-Chen Lyu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Allergy
Adolescent medicine.medical_treatment Amino Acid Motifs Juglans medicine.disease_cause Immunoglobulin E 01 natural sciences Young Adult 03 medical and health sciences 0302 clinical medicine Allergen Western blot Botany medicine Chemical Precipitation Humans Food science RNA Processing Post-Transcriptional Child Ammonium sulfate precipitation Plant Proteins Juglans spp Protease biology medicine.diagnostic_test Chemistry Circular Dichroism Immune Sera 010401 analytical chemistry General Chemistry Allergens Antigens Plant biology.organism_classification medicine.disease 0104 chemical sciences 030228 respiratory system Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel Nut Hypersensitivity General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:454-462 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.6b04387 |
| Popis: | Tree nuts as a group cause a significant number of fatal anaphylactic reactions to foods. Walnuts (Juglans spp.) are one of the leading causes of allergic reactions to tree nuts in the U.S. and Japan. The purpose of this study was to purify and characterize potential food allergens from black walnut. Here, we report the isolation of the black walnuts allergen Jug n 4 (an 11S globulin) by ammonium sulfate precipitation, hydrophobic interaction, and size exclusion chromatography. Reducing SDS-PAGE analysis indicated that purified Jug n 4 consists of three major bands. N-Terminal sequencing data of these bands indicated that they were the results of a post-transcriptional protease cleavage of the mature protein at a site that consists of a known conserved protease recognition motif, NGXEET. Western blot experiments revealed that 32% of the sera from 25 patients with double-blind, placebo-controlled clinical walnut allergy contained IgE antibodies that recognized Jug n 4, indicating that it is a walnut allergen. Identifying this and additional allergens may facilitate the understanding of the allergenicity of seed storage proteins in tree nuts and their cross-reactivity. |
| Databáze: | OpenAIRE |
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