Extensive Contamination of Water with Saxitoxin Near the Dam of the Irkutsk Hydropower Station Reservoir (East Siberia, Russia)
| Autor: | Elena Eletskaya, M.A. Grachev, Maria Ivacheva, Tatyana Pogadaeva, Viktor Minaev, Irina A Tikhonova, E. G. Sorokovikova, Vladimir A. Shagun, Aleksandr Galkin, Anton V. Kuzmin, Andrey Ivanov, G. A. Fedorova, V. B. Smirnov, E. V. Sukhanova, Olga I. Belykh, Olga Netsvetayeva, M. Suslova, Ilya N. Zubkov |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cyanobacteria DNA Bacterial Health Toxicology and Mutagenesis Dolichospermum lemmermannii saxitoxin lcsh:Medicine 010501 environmental sciences Toxicology 01 natural sciences cyanobacteria Article 03 medical and health sciences chemistry.chemical_compound hydrochemical analysis Nutrient Water Supply Lake Baikal 0105 earth and related environmental sciences Heterocyst Saxitoxin Biomass (ecology) biology lcsh:R Contamination biology.organism_classification Monitoring program HPLC-MS Siberia Lakes 030104 developmental biology chemistry Irkutsk reservoir Environmental chemistry Environmental science ELISA Bloom Water Pollutants Chemical Environmental Monitoring Power Plants |
| Zdroj: | Toxins, Vol 10, Iss 10, p 402 (2018) Toxins Volume 10 Issue 10 |
| ISSN: | 2072-6651 |
| Popis: | An area of discolored water 50 m wide and 30 m long was found in September 2017 close to the dam of the Irkutsk hydroelectric power station. Water from this spot was sampled for investigation in the present study. Microscopic analysis revealed that the suspended matter in the sample was composed of clumps of filaments, vegetative cells, akinetes and heterocysts that formed short filaments and solitary cells. This matter was found to consist of partially degraded cells of the cyanobacterium Dolichospermum lemmermannii. Nucleotide sequencing of DNA isolated from the biomass revealed the presence of the sxtA gene which is involved in the synthesis of saxitoxin. Water from the polluted area contained 600 ± 100 &mu g L&minus 1 saxitoxin as measured by HPLC-MS with pre-column modification of the toxin with 2,4-dinitrophenylhydrazine. Immunoassay analysis (ELISA) showed a concentration of saxitoxins in the water of 2900 ± 900 &mu 1. Hydrochemical and microbiological analyses suggested the contaminated area appeared as a result of a D. lemmermannii bloom, followed by its decay and release of saxitoxin and nutrients. The present paper describes the results of a case study. Better understanding of the phenomenon will depend on the possibility to perform implementation of a large-scale monitoring program. |
| Databáze: | OpenAIRE |
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