Protein kinase C expression in salivary gland acinar epithelial cells in non-obese diabetic mice, an experimental model for Sjögren's syndrome
| Autor: | J. Törnwall, Mika Hukkanen, Yrjö T. Konttinen, Tensing Ek, H. S. Fox, Jian Ma, Tian-Fang Li |
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| Rok vydání: | 2003 |
| Předmět: |
Gene isoform
medicine.medical_specialty Immunology Nod PKC alpha Salivary Glands Immunoenzyme Techniques Mice stomatognathic system Rheumatology Mice Inbred NOD Internal medicine Immunology and Allergy Medicine Animals Protein kinase C Protein Kinase C NOD mice Mice Inbred BALB C Salivary gland business.industry Epithelial Cells medicine.disease Sialadenitis Epithelium Isoenzymes Disease Models Animal Endocrinology medicine.anatomical_structure Diabetes Mellitus Type 1 Sjogren's Syndrome business Signal Transduction |
| Zdroj: | Rheumatology international. 25(1) |
| ISSN: | 0172-8172 |
| Popis: | We planned to investigate the expression of protein kinase C (PKC) isoforms in acinar epithelial cells of salivary glands in the non-obese diabetic (NOD) mouse to find out if they develop changes of the PKC system like those seen in the human counterpart, i.e. in Sjogren's syndrome. Parotid, submandibular, and sublingual glands from NOD and control BALB/c mice were stained with a panel of monoclonal antibodies directed against conventional (alpha, beta, and gamma), novel (delta, epsilon, and theta), and atypical (lambda and iota) PKC isoforms using the streptavidin/HRP method. Similarly to human labial salivary glands, acinar epithelial cells of the healthy control BALB/c mice contained two of the conventional PKC isoforms, alpha and beta. Acinar and ductal epithelial cells also contained the atypical PKC isoforms lambda and iota. PKC isoforms gamma, delta, epsilon, and theta were not found. NOD mice which displayed focal sialadenitis contained the same conventional and atypical PKC isoforms. The acinar cells in NOD mice, in contrast to the Sjogren's syndrome patients, did not lack PKC alpha or beta. On the contrary, PKC alpha and beta staining was stronger than in the control BALB/c mice. The present results demonstrate that both conventional and atypical PKC isoforms participate in the salivary epithelial cell biology and that there are mouse strain-associated and/or disease state-associated changes in their expression. The lack of PKC alpha and beta isoforms found in Sjogren's syndrome was not reproduced in NOD mice, which discloses one more difference between the human disease and its NOD mouse model. |
| Databáze: | OpenAIRE |
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