Effect of lithium carbonate on autophagy and proliferative activity of isolated hepatocytes
| Autor: | Raushan Dossymbekova, K. O. Sharipov, Asem Kydyrbaeva, Gulzhan Taneyeva, Zina Tungushbayeva, N. P. Bgatova, Anastasiya Solovieva |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cell Biophysics Cell Separation Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lithium Carbonate Autophagy medicine Animals Inducer Rats Wistar Molecular Biology Cells Cultured Cell Proliferation Cell Cycle Lithium carbonate Cell Biology Cell cycle Cell biology Transplantation 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Hepatocyte Hepatocytes Homeostasis |
| Zdroj: | Biochemical and Biophysical Research Communications. 528:343-346 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.03.057 |
| Popis: | The hepatocytes were cultivated in the presence of lithium carbonate (LC) for drugs testing or possible source for transplantation in the treatment of hereditary or terminal liver diseases. The LC, as an inducer of autophagy, is a promising drug for maintaining cell homeostasis and has a significant effect on the ultrastructural organization of hepatocyte cells. Within current investigation, new mechanisms of the biological effects of lithium and the ultrastructural analysis of the primary culture of hepatocytes were studied via flow cytofluorometry, light, and electron microscopy methods. Obtained results demonstrate the absence of the toxic effect of 5 mM of LC on the primary hepatocyte culture. In addition, LC does not block the cell cycle at the G0/G1 stage after 24 h of hepatocyte cultivation and promotes the preservation of their viability by 48 h of the experiment. Moreover, LC does not stimulate hepatocyte apoptosis, induces autophagy and the preserves the proliferative activity of hepatocytes. |
| Databáze: | OpenAIRE |
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