Synthesis and immunogenicity of hepatitis B virus envelope antigen expressed by recombinant vaccinia virus. Finding of retention signal in the C-terminal portion of the preS1 domain of subtype adyw
| Autor: | Š. Němečková, Eva Hamsikova, V. Vonka, D. Kunke, J. Smrt, L. Kutinová, H. Závadová, M. Press |
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| Rok vydání: | 1991 |
| Předmět: |
Gene Expression Regulation
Viral HBsAg Hepatitis B virus Molecular Sequence Data Vaccinia virus Protein Sorting Signals medicine.disease_cause Antibodies Viral Virus Cell Line Mice Antigen Virology medicine Animals Poxviridae Orthopoxvirus Cloning Molecular Protein Precursors Hepatitis B Surface Antigens biology Base Sequence Immunogenicity General Medicine biology.organism_classification Molecular biology Precipitin Tests Rats Blotting Southern Hepadnaviridae DNA Viral Immunization Peptides Plasmids |
| Zdroj: | Archives of virology. 121(1-4) |
| ISSN: | 0304-8608 |
| Popis: | Five different recombinant vaccinia viruses expressing the envelope antigen of hepatitis B virus (HBsAg) under the control of the P7·5 promoter were constructed. Cell cultures infected with some of the recombinant viruses synthesized both middle (M) and major surface (S) protein of HBsAg. It was shown that the length of the nontranslated sequence preceding preS2-ATG influenced the extracellular or intracellular HBV antigen distribution and the preS2:S antigen ratio. Some recombinants synthesized an M protein that was enlarged by additional 35 amino acids of preS1 domain and was entirely retained within the infected cells. Antibody responses to the S and preS2 antigens in mice revealed significant differences in the immunogenicity of individual recombinants. |
| Databáze: | OpenAIRE |
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