AIEC colonization and pathogenicity: influence of previous antibiotic treatment and preexisting inflammation
Autor: | Cécile Vignal, Pierre Desreumaux, Antoine Cortot, Luc Dubreuil, Maryline Drouet, Madjid Djouina, Christel Neut, Elisabeth Singer |
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Rok vydání: | 2011 |
Předmět: |
Male
medicine.drug_class Antibiotics Blotting Western Nod2 Signaling Adaptor Protein Inflammation Ileum Biology Real-Time Polymerase Chain Reaction Bacterial Adhesion Microbiology Mice NOD2 medicine Escherichia coli Immunology and Allergy Animals Humans Colonization RNA Messenger Colitis Escherichia coli Infections Mice Knockout Reverse Transcriptase Polymerase Chain Reaction Dextran Sulfate Gastroenterology medicine.disease digestive system diseases Anti-Bacterial Agents Intestines Mice Inbred C57BL medicine.anatomical_structure Bacterial Translocation Knockout mouse Immunology Female medicine.symptom Dysbiosis |
Zdroj: | Inflammatory bowel diseases. 18(10) |
ISSN: | 1536-4844 |
Popis: | Background: Inflammatory bowel diseases (IBD) patients are abnormally colonized by adherent-invasive Escherichia coli (AIEC). NOD2 gene mutations impair intracellular bacterial clearance. We evaluated the impact of antibiotic treatment on AIEC colonization in wildtype (WT) and NOD2 knockout mice (NOD2KO) and the consequences on intestinal inflammation. Methods: After 3 days of antibiotic treatment, mice were infected for 2 days with 109 CFU AIEC and sacrificed 1, 5, and 60 days later. In parallel, mice were challenged with AIEC subsequent to a dextran sodium sulfate (DSS) treatment and sacrificed 9 days later. Ileum, colon, and mesenteric tissues were sampled for AIEC quantification and evaluation of inflammation. Results: Without antibiotic treatment, AIEC was not able to colonize WT and NOD2KO mice. Compared with nontreated animals, antibiotic treatment led to a significant increase in ileal and colonic colonization of AIEC in WT and/or NOD2KO mice. Persistent AIEC colonization was observed until day 5 only in NOD2KO mice, disappearing at day 60. Mesenteric translocation of AIEC was observed only in NOD2KO mice. No inflammation was observed in WT and NOD2KO mice treated with antibiotics and infected with AIEC. During DSS-induced colitis, colonization and persistence of AIEC was observed in the colon. Moreover, a dramatic increase in clinical, histological, and molecular parameters of colitis was observed in mice infected with AIEC but not with a commensal E. coli strain. Conclusions: Antibiotic treatment was necessary for AIEC colonization of the gut and mesenteric tissues and persistence of AIEC was dependent on NOD2. AIEC exacerbated a preexisting DSS-induced colitis in WT mice. (Inflamm Bowel Dis 2012) |
Databáze: | OpenAIRE |
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