Dual activities of galectin-3 in human prostate cancer: tumor suppression of nuclear galectin-3 vs tumor promotion of cytoplasmic galectin-3
Autor: | Frédéric van den Brûle, Vincent Castronovo, Marc Bracke, Stéphane Califice |
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Rok vydání: | 2004 |
Předmět: |
Male
Cancer Research medicine.medical_specialty Cytoplasm animal structures Galectin 3 Chick Embryo Biology medicine.disease_cause Prostate cancer Internal medicine LNCaP otorhinolaryngologic diseases Genetics medicine Animals Humans Neoplasm Invasiveness Molecular Biology Cellular localization DNA Primers Cell Nucleus Base Sequence Cancer Prostatic Neoplasms medicine.disease Immunohistochemistry stomatognathic diseases Endocrinology Tumor progression Cancer cell Cancer research Androgens Tumor promotion Carcinogenesis |
Zdroj: | Oncogene. 23(45) |
ISSN: | 0950-9232 |
Popis: | Galectin-3, a multifunctional lectin, is involved during cancer progression. Previous observations showed that both cytosolic expression and nuclear exclusion of galectin-3 in human prostate cancer cells were associated to progression of the disease. In this study, we examined the biological roles of galectin-3 when expressed either in the nucleus or in the cytosol. LNCaP, a galectin-3-negative human prostate cancer cell line, was used to generate transfectants expressing galectin-3 either in the nucleus or in the cytosol. No changes in cell morphology, proliferation, attachment to laminin-1 or androgen dependency were observed. Cytoplasmic galectin-3 induced significantly increased Matrigel invasion, anchorage-independent growth and in vivo tumor growth and angiogenesis, and decreased inducible apoptosis. Surprisingly, nuclear galectin-3 affected these parameters in an opposite fashion with an overall antitumoral activity. Thus, our study demonstrates that galectin-3 exerts opposite biological activities according to its cellular localization: nuclear galectin-3 plays antitumor functions and cytoplasmic galectin-3 promotes tumor progression. |
Databáze: | OpenAIRE |
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