A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves
| Autor: | Stefan Schuechner, Gregory C Nordmann, David A. Keays, Robert Heinen, Lukas Landler, Egon Ogris, Erich Pascal Malkemper, Lyubov Ushakova, Simon Nimpf |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Auditory Pathways medicine.drug_class lcsh:Medicine Biology Monoclonal antibody Article Birds 03 medical and health sciences Antibodies Monoclonal Murine-Derived Mice 0302 clinical medicine Mouse monoclonal antibody medicine Premovement neuronal activity Animals lcsh:Science Columbidae Zebra finch Transcription factor Early Growth Response Protein 1 Neurons Multidisciplinary lcsh:R Brain Immunohistochemistry 3. Good health Cell biology 030104 developmental biology biology.protein lcsh:Q Antibody Immediate early gene 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2045-2322 |
| Popis: | The transcription factor ZENK is an immediate early gene that has been employed as a surrogate marker to map neuronal activity in the brain. It has been used in a wide variety of species, however, commercially available antibodies have limited immunoreactivity in birds. To address this issue we generated a new mouse monoclonal antibody, 7B7-A3, raised against ZENK from the rock pigeon (Columba livia). We show that 7B7-A3 labels clZENK in both immunoblots and histological stainings with high sensitivity and selectivity for its target. Using a sound stimulation paradigm we demonstrate that 7B7-A3 can detect activity-dependent ZENK expression at key stations of the central auditory pathway of the pigeon. Finally, we compare staining efficiency across three avian species and confirm that 7B7-A3 is compatible with immunohistochemical detection of ZENK in the rock pigeon, zebra finch, and domestic chicken. Taken together, 7B7-A3 represents a useful tool for the avian neuroscience community to map functional activity in the brain. |
| Databáze: | OpenAIRE |
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