A New in Vivo and in Vitro B cell Lymphoma Model, π-BCL1
| Autor: | William Howatt, Bridget Wilkins, Timothy M Illidge, Fiona Ross, Jamie Honeychurch, Mark S. Cragg |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Lymphoma B-Cell CD30 Fluorescent Antibody Technique In Vitro Techniques Biology Immunophenotyping Mice In vivo hemic and lymphatic diseases Tumor Cells Cultured medicine Animals Radiology Nuclear Medicine and imaging B-cell lymphoma Lymphoma Follicular Pharmacology Mice Inbred BALB C Cell growth Large cell DNA Neoplasm General Medicine medicine.disease In vitro Lymphoma Disease Models Animal Liver Oncology Karyotyping Antigens Surface Female Spleen |
| Zdroj: | Cancer Biotherapy and Radiopharmaceuticals. 15:571-580 |
| ISSN: | 1557-8852 1084-9785 |
| DOI: | 10.1089/cbr.2000.15.571 |
| Popis: | We report a new variant of the BCL1 syngeneic mouse B-cell lymphoma model, which we have called pi-BCL1. pi-BCL1 can be established as a syngeneic tumor in BALB/c mice. Tumors can be removed, prepared and easily grown in liquid culture and subsequently transferred back successfully as syngeneic tumors. As a syngeneic tumor pi-BCL1 behave more like a lymphoma with solid tumor masses, than a chronic lymphocytic leukaemia of the original BCL1 model. The immunophenotype and the growth characteristics of the pi-BCL1 and BCL1 tumors appear very similar. Cytologically, pi-BCL1 appears to be a transformation from a small lymphocytic lymphoma to a more diffuse large cell centroblast-like higher-grade lymphoma. We are not aware of any previous reports of such transformation events in a syngeneic animal model of B cell lymphoma. We believe pi-BCL1 provides a useful new tool for the study of B cell lymphoma in vitro and in vivo and enables reduced numbers of tumor passage in mice. |
| Databáze: | OpenAIRE |
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