Silica nanoparticles actively engage with mesenchymal stem cells in improving acute functional cardiac integration
| Autor: | Valentina Turinetto, Claudia Giachino, Gabriele Alberto, Jasmin Popara, Raffaella Rastaldo, Pasquale Pagliaro, Emanuela Vitale, Gianmario Martra, Ambra Iannuzzi, Dorotea Roggio, Lisa Accomasso, Clara Gallina, Federico Catalano, Stefania Raimondo |
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| Rok vydání: | 2018 |
| Předmět: |
cell adhesion
gap junction heart mesenchymal stem cell regeneration silica nanoparticle Bioengineering Medicine (miscellaneous) Biomedical Engineering Materials Science (all) Development3304 Education 0301 basic medicine media_common.quotation_subject Development Education Focal adhesion 03 medical and health sciences Animals Humans Myocytes Cardiac General Materials Science Cell adhesion Internalization media_common Focal Adhesions Chemistry Myocardium Regeneration (biology) Mesenchymal stem cell Gap junction Cell Differentiation Mesenchymal Stem Cells Adhesion 3304 Silicon Dioxide equipment and supplies Coculture Techniques In vitro Rats Cell biology 030104 developmental biology Gene Expression Regulation Connexin 43 Nanoparticles |
| Zdroj: | Nanomedicine. 13:1121-1138 |
| ISSN: | 1748-6963 1743-5889 |
| Popis: | Aim: To assess functional effects of silica nanoparticles (SiO2-NPs) on human mesenchymal stem cell (hMSC) cardiac integration potential. Methods: SiO2-NPs were synthesized and their internalization effects on hMSCs analyzed with particular emphasis on interaction of hMSCs with the cardiac environment Results: SiO2-NP internalization affected the area and maturation level of hMSC focal adhesions, accounting for increased in vitro adhesion capacity and augmented engraftment in the myocardial tissue upon cell injection in infarcted isolated rat hearts. SiO2-NP treatment also enhanced hMSC expression of Connexin-43, favoring hMSC interaction with cocultured cardiac myoblasts in an ischemia-like environment. Conclusion: These findings provide strong evidence that SiO2-NPs actively engage in mediating biological effects, ultimately resulting in augmented hMSC acute cardiac integration potential. |
| Databáze: | OpenAIRE |
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