Stem cell induction and plant regeneration are affected by medium components in maca (Lepidium meyenii Walp)

Autor: Danial Kahrizi, Atefeh Fahimi Farr, Zeinab Chaghakaboodi, Mozafar Khazaei, Esra Ucar Sozmen, Hulya Dogan
Rok vydání: 2023
Předmět:
Zdroj: Molecular Biology Reports. 50:4187-4192
ISSN: 1573-4978
0301-4851
DOI: 10.1007/s11033-023-08319-2
Popis: Selection, propagation and conservation of important genotypes are important in medicinal-industrial plants. Nowadays, using tissue culture and regeneration techniques of medicinal plants under in vitro conditions has been able to proliferate medicinal plants widely, which is much higher than traditional methods of vegetative propagation. Maca (Lepidium meyenii), is an industrial plant whose root is the usable part. Maca has valuable medicinal effects such as sexual enhancement and reproductive power, infertility treatment, improved sperm count and quality, anti-stress, osteoporosis prevention and more. This study was conducted to induce callus and regeneration of Maca. First, MS medium supplemented with different concentrations of Kinetin, NAA and 2,4-D (0.5, 1 and 2 μM respectively) and control were compared for callus induction from root and leaves. After 38 days of incubation, the first callus appeared, after 50 days of callus induction and after 79 days regeneration occurred. The callus induction experiment was performed for the study of the effect of three explants (leaf, stem and root) and seven hormone levels. The regeneration experiment was carried out by studying the effect of three explants (leaf, stem and root) on eight levels of the hormone. The results of data analysis on callus induction showed that the effects of explants, hormones and their interactions on callus induction percentage were highly significant but not significant on callus growth rate. The results of regression analysis showed that explants, hormones and their interactions had no significant effect on regeneration percentage.
Databáze: OpenAIRE
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