RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats
| Autor: | Marcela C. Scalon, Giane Regina Paludo, Christine Souza Martins, Franciele Schlemmer, Gabriel Ginani Ferreira, Ricardo Titze-de-Almeida |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
CATS General Veterinary Reverse Transcriptase Polymerase Chain Reaction 040301 veterinary sciences 04 agricultural and veterinary sciences Biology Prognosis 0403 veterinary science Andrology MicroRNAs 03 medical and health sciences Circulating MicroRNA Real-time polymerase chain reaction Gene expression microRNA Cats Animals Biomarker (medicine) Gene silencing Brief Reports Biomarkers 030304 developmental biology Blood sampling |
| Zdroj: | J Vet Diagn Invest |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/10406387211034843 |
| Popis: | MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level by silencing targeted messenger RNA (mRNA). Most studies concerning miRNA expression use solid tissue samples. However, circulating miRNAs from different body fluids have recently emerged as diagnostic and prognostic molecules, given that they hold informative value and have increased stability in cell-free form. Blood sampling of cats can be challenging given their small body size and because they often experience distress when handled. We quantified miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of 10 healthy domestic cats. Our RT-rtPCR procedure used 100 µL of either plasma or serum samples as sources of biomarker molecules. However, serum provided higher amounts of miRNA than plasma samples, with a p |
| Databáze: | OpenAIRE |
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