Effect of γ-Irradiation on Expression of Tight and Adherens Junction Protein mRNA on In Vitro Blood–Brain Barrier Model
| Autor: | A. V. Golanov, Vladimir P. Chekhonin, Ya. A. Zorkina, N. E. Volgina, P. A. Mel’nikov, Alexander Potapov, Gorlachev Ge |
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| Rok vydání: | 2014 |
| Předmět: |
Gene Expression
Biology γ irradiation Blood–brain barrier General Biochemistry Genetics and Molecular Biology Tight Junctions Adherens junction In vivo Human Umbilical Vein Endothelial Cells medicine Humans Claudin-5 Cells Cultured beta Catenin Messenger RNA Tight junction Adherens Junctions General Medicine Coculture Techniques In vitro Cell biology medicine.anatomical_structure Blood-Brain Barrier Gamma Rays Permeability (electromagnetism) Zonula Occludens-1 Protein |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 158:127-136 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-014-2708-5 |
| Popis: | We studied the effect of γ-irradiation on HUVEC endothelial cells co-cultured with allogeneic astrocytes. This 2D in vitro model of the blood-brain barrier has the same parameters as cerebral microvascular endothelial cells forming the blood-brain barrier and allows reproducing its functions in vivo. Dose-dependent changes in cell morphology and violation of monolayer integrity were observed. Real-time PCR and immunocytochemical analysis revealed changes in the expression of tight (ZO-1, claudin-5) and adherens junction protein (vascular endothelial cadherin, β-catenin) mRNA. Expression of tight and adherens junction proteins mRNA decreased in 2, 24, and 48 h after irradiation in doses of 2, 4, and 6 Gy. Significant dose-dependent changes were found only for β-catenin mRNA expression in 2 h after exposition. This model of blood-brain barrier in vitro can be used for studying the molecular mechanisms regulating permeability of cerebral endothelium under normal conditions and after pathological exposures, e.g. γ-irradiation. |
| Databáze: | OpenAIRE |
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