Quorum Quenching in a Novel Acinetobacter sp. XN-10 Bacterial Strain against Pectobacterium carotovorum subsp. carotovorum
| Autor: | Sandhya Mishra, Lian-Hui Zhang, Tian Ye, Luo Qingqing, Shaohua Chen, Zhang Yiyin, Pankaj Bhatt, Wenping Zhang, Fan Xinghui |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Cell signaling N-acyl homoserine lactones Pectobacterium carotovorum Microbiology Article 03 medical and health sciences Virology Pectobacterium carotovorum subsp. carotovorum biocontrol lcsh:QH301-705.5 Gene 030304 developmental biology chemistry.chemical_classification 0303 health sciences Strain (chemistry) biology 030306 microbiology food and beverages quorum sensing biochemical phenomena metabolism and nutrition biology.organism_classification Quorum sensing lcsh:Biology (General) Acinetobacter sp. XN-10 chemistry Quorum Quenching quorum quenching Lactone Bacteria |
| Zdroj: | Microorganisms Volume 8 Issue 8 Microorganisms, Vol 8, Iss 1100, p 1100 (2020) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8081100 |
| Popis: | Quorum sensing (QS) is a cell density-dependent mechanism that regulates the expression of specific genes in microbial cells. Quorum quenching (QQ) is a promising strategy for attenuating pathogenicity by interfering with the QS system of pathogens. N-Acyl-homoserine lactones (AHLs) act as signaling molecules in many Gram-negative bacterial pathogens and have received wide attention. In this study, a novel, efficient AHL-degrading bacterium, Acinetobacter sp. strain XN-10, was isolated from agricultural contaminated soil and evaluated for its degradation efficiency and potential use against QS-mediated pathogens. Strain XN-10 could effectively degrade N-(3-oxohexanoyl)-L-homoserine lactone (OHHL), N-hexanoyl-L-homoserine lactone (C6HSL), N-(3-oxododecanoyl)-L-homoserine lactone (3OC12HSL), and N-(3-oxooctanoyl)-L-homoserine lactone (3OC8HSL), which all belong to the AHL family. Analysis of AHL metabolic products by gas chromatography&ndash mass spectrometry (GC-MS) led to the identification of N-cyclohexyl-propanamide, and pentanoic acid, 4-methyl, methyl ester as the main intermediate metabolites, revealing that AHL could be degraded by hydrolysis and dehydroxylation. All intermediates were transitory and faded away without any non-cleavable metabolites at the end of the experiment. Furthermore, strain XN-10 significantly attenuated the pathogenicity of Pectobacterium carotovorum subsp. carotovorum (Pcc) to suppress tissue maceration in carrots, potatoes, and Chinese cabbage. Taken together, our results shed light on the QQ mechanism of a novel AHL-degrading bacterial isolate, and they provide useful information which show potential for biocontrol of infectious diseases caused by AHL-dependent bacterial pathogens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|