Association of goat (Capra hircus) CD4 gene exon 6 polymorphisms with ability of sperm internalizing exogenous DNA
Autor: | Yongju Zhao, Mingju Yu, Jingsheng Fan, Lingbin Wang, Shuangyan Zheng |
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Rok vydání: | 2010 |
Předmět: |
Male
Genotype Molecular Sequence Data Breeding Biology Transfection Polymerase Chain Reaction Polymorphism Single Nucleotide chemistry.chemical_compound Exon Gene Frequency Semen Molecular marker Genetics Capra hircus Animals Least-Squares Analysis Molecular Biology Gene Alleles Genetic Association Studies Polymorphism Single-Stranded Conformational Sperm plasma membrane Base Sequence Goats DNA Exons General Medicine Spermatozoa Sperm Molecular biology Endocytosis genomic DNA chemistry CD4 Antigens Exogenous DNA Sequence Alignment |
Zdroj: | Molecular Biology Reports. 38:1621-1628 |
ISSN: | 1573-4978 0301-4851 |
Popis: | As one of the transport systems on the sperm plasma membrane, CD4 molecule plays a distinct role in the process of sperm/DNA interaction. This makes it possible to explain the mechanism of sperm-mediated gene transfer (SMGT), which at present is still a mystery in this area. In this study, seminal samples of 60 individuals from seven breed bucks were collected to detect the ability of sperm in internalizing exogenous DNA, and genomic DNA from 147 individual blood samples (including 60 bucks referred ahead) were extracted to test the polymorphisms of CD4 genes by using PCR-SSCP technique. Then the correlation between them was evaluated. The results showed that: (1) it was a novel finding that breed-dependence of exogenous DNA binding to goat spermatozoa. There was the most significant difference among the buck breeds of sperm in binding exogenous DNA (F((6, 53)) = 4.811, P = 0.001) and in internalizing them into nuclei (F((6, 53)) = 4.587, P = 0.001). The ability of Lezhi Black goat was the lowest (P0.01) among the seven breeds. (2) There was no significant correlation between the ability of sperm in internalizing exogenous DNA and each semen quality parameter (P0.05). (3) In particular, three single-nucleotide polymorphisms (SNP) were described and there was one SNP (G/A(700)) of CD4 gene that made G234R substitution in the amino acid sequence of CD4 molecule. Nanjiang Yellow goat and Lezhi Black goat had higher hereditary variation compared with other breeds. (4) CD4 polymorphisms were highly associated with the ability of sperm in internalizing exogenous DNA. The SNP of Caprine CD4 gene exon 6 might be an important molecular marker of the ability to internalize exogenous DNA into sperm. |
Databáze: | OpenAIRE |
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