High heterogeneity, mixed infections and new genotypes in human congenital toxoplasmosis cases in the mega-metropolis of Central Mexico
| Autor: | Lorena Hernández-Delgado, Dolores Correa, Claudia Patricia Rico-Torres, Ricardo Figueroa-Damián, Luis Fernando Valenzuela-Moreno, Valeria Gómez-Toscano, Héctor Luna-Pastén, Mónica Patricia Escobedo-Torres |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Genotype Veterinary (miscellaneous) 030231 tropical medicine Biology Parasite load Parasite Load Toxoplasmosis Congenital 03 medical and health sciences Young Adult 0302 clinical medicine parasitic diseases Parasite hosting Animals Humans Allele Genotyping Mexico Alleles Genetic diversity Coinfection Toxoplasma gondii Genetic Variation biology.organism_classification Virology 030104 developmental biology Infectious Diseases Insect Science Cohort Parasitology Female Polymorphism Restriction Fragment Length |
| Zdroj: | Acta tropica. 178 |
| ISSN: | 1873-6254 |
| Popis: | Mexico presents high prevalence of Toxoplasma gondii infection, including the congenital form, but there are few data about the genetic diversity of the parasite, so we attempted parasite isolation and genotyping in nine mother/children pairs with congenital toxoplasmosis (CT), living in the Valley of Mexico, who were part of a 30 cases cohort that started 12 years ago. They were recruited through research projects which included pre- and postnatal screening of congenital infections or directly CT, and cases referred to INP for management because they had clinical abnormalities. Genotyping was performed by PCR-RFLP of SAG1, SAG2, SAG3, BTUB GRA6, c22-8, c29-2, L358, PK1 and Apico markers, followed by sequencing. Sixty seven percent of samples were typed for the SAG3 locus, 39% for Apico and 33% for BTUB, while Alt. SAG2, GRA6 and c29-2 types could be labelled in less cases. Type I alleles predominated, followed by II and III. We isolated the first strain obtained from humans in Mexico and found three genotypes not previously found in the world. The presence of ToxoDB#10 clonal type was documented in one pair, as well as mixed infections in five mothers. No relation of genotype or parasite load with clinical signs was found. In conclusion, we encountered great genetic diversity and mixed T. gondii infections among mother/children pairs with congenital toxoplasmosis in the mega-metropolis of the Valley of Mexico. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect