Mechanisms driving neutrophil-induced t-cell immunoparalysis in ovarian cancer
| Autor: | Viviana P. Ferreira, Ilse Jongerius, Kelly L. Singel, Kunle Odunsi, Stephanie L. Silva-Del Toro, Thejaswini Giridharan, Anm Nazmul H. Khan, Jason Ricciuti, Kirsten B. Moysich, Kaitlyn Howard, Emese Zsiros, Kevin H. Eng, Tiffany R. Emmons, Brahm H. Segal, James A. Lederer, Sora Suzuki, Cathelijn E.M. Aarts, Holger Sellner, Taco W. Kuijpers, Mieke C. Brouwer, Steven M. Holland, Joerg Eder, Michael B. Yaffe, Ivy L. Debreceni, Anna Schubart, Sanjay Ram, Lee-Ann H. Allen |
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| Přispěvatelé: | Graduate School, CCA - Cancer biology and immunology, Neurology, ANS - Neuroinfection & -inflammation, ARD - Amsterdam Reproduction and Development, Paediatric Infectious Diseases / Rheumatology / Immunology, Landsteiner Laboratory, AII - Cancer immunology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
0301 basic medicine Hypersegmented neutrophil Cancer Research Trogocytosis Neutrophils T-Lymphocytes T cell Primary Cell Culture Immunology Lymphocyte Activation Neutrophil Activation Young Adult 03 medical and health sciences 0302 clinical medicine Immune system Tumor Microenvironment medicine Humans Cells Cultured PI3K/AKT/mTOR pathway Ovarian Neoplasms Tumor microenvironment NADPH oxidase biology Chemistry NFAT Middle Aged Cell biology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Female Tumor Escape |
| Zdroj: | Cancer immunology research, 9(7), 790-810. American Association for Cancer Research Inc. |
| ISSN: | 2326-6066 |
| Popis: | T-cell activation and expansion in the tumor microenvironment (TME) are critical for antitumor immunity. Neutrophils in the TME acquire a complement-dependent T-cell suppressor phenotype that is characterized by inhibition of T-cell proliferation and activation through mechanisms distinct from those of myeloid-derived suppressor cells. In this study, we used ascites fluid supernatants (ASC) from patients with ovarian cancer as an authentic component of the TME to evaluate the effects of ASC on neutrophil function and mechanisms for neutrophil-driven immune suppression. ASC prolonged neutrophil life span, decreased neutrophil density, and induced nuclear hypersegmentation. Mass cytometry analysis showed that ASC induced 15 distinct neutrophil clusters. ASC stimulated complement deposition and signaling in neutrophils, resulting in surface mobilization of granule constituents, including NADPH oxidase. NADPH oxidase activation and phosphatidylserine signaling were required for neutrophil suppressor function, although we did not observe a direct role of extracellular reactive oxygen species in inhibiting T-cell proliferation. Postoperative surgical drainage fluid also induced a complement-dependent neutrophil suppressor phenotype, pointing to this effect as a general response to injury. Like circulating lymphocytes, ASC-activated neutrophils caused complement-dependent suppression of tumor-associated lymphocytes. ASC-activated neutrophils adhered to T cells and caused trogocytosis of T-cell membranes. These injury and signaling cues resulted in T-cell immunoparalysis characterized by impaired NFAT translocation, IL2 production, glucose uptake, mitochondrial function, and mTOR activation. Our results demonstrate that complement-dependent priming of neutrophil effector functions in the TME induces a T-cell nonresponsiveness distinct from established checkpoint pathways and identify targets for immunotherapy.See related Spotlight by Cassatella, p. 725. |
| Databáze: | OpenAIRE |
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