Human Stem Cell-Derived Retinal Pigment Epithelial Cells as a Model for Drug Screening and Pre-Clinical Assays Compared to ARPE-19 Cell Line
| Autor: | Juliana Lott Carvalho, Armando Silva-Cunha, Márcio Bittar Nehemy, Mayara Rodrigues Brandão de Paiva, Gracielle Ferreira Andrade, Carolina Reis de Oliveira, Sílvia Ligório Fialho, Marcela Coelho Silva Ribeiro, Dawidson Assis Gomes, Alfredo M. Goes |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
education.field_of_study
Human embryonic stem cell derived-retinal pigmented epithelial cells Population Cell Biology Biology Embryonic stem cell Cell therapy chemistry.chemical_compound chemistry In vitro model Cell culture Teriflunomide Cancer research Original Article sense organs Stem cell Induced pluripotent stem cell education Immortalised cell line ARPE-19 Developmental Biology In vitro toxicology |
| Zdroj: | International Journal of Stem Cells |
| ISSN: | 2005-5447 2005-3606 |
| Popis: | Background and objectives Eye diseases have a high socioeconomic impact on society and may be one of the fields in which most stem cell-related scientific accomplishments have been achieved recently. In this context, human Pluripotent Stem Cell (hPSC) technology arises as an important tool to produce and study human Embryonic Stem cell derived-Retinal Pigmented Epithelial Cells (hES-RPE) for several applications, such as cell therapy, disease modeling, and drug screening. The use of this technology in pre-clinical phases attends to the overall population desire for animal-free product development. Here, we aimed to compare hES-RPE cells with ARPE-19, one of the most commonly used retinal pigmented epithelial immortalized cell lines. Methods and results Functional, cellular and molecular data obtained suggest that hES-RPE cells more closely resembles native RPEs compared to ARPE-19. Furthermore, hES-RPE revealed an interesting robustness when cultured on human Bruch's membrane explants and after exposure to Cyclosporine (CSA), Sirolimus (SRL), Tacrolimus (TAC), Leflunomide (LEF) and Teriflunomide (TER). On these conditions, hES-RPE cells were able to survive at higher drug concentrations, while ARPE-19 cell line was more susceptible to cell death. Conclusions Therefore, hES-RPEs seem to have the ability to incur a broader range of RPE functions than ARPE-19 and should be more thoroughly explored for drug screening. |
| Databáze: | OpenAIRE |
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