Wheat germ agglutinin and Lens culinaris agglutinin sensitized anisotropic silver nanoparticles in detection of bacteria: A simple photometric assay
| Autor: | Olga D. Hendrickson, Mariam V. Mikaelyan, Boris B. Dzantiev, Gayane G. Poghosyan, Vardan K. Gasparyan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Staphylococcus aureus
Silver Wheat Germ Agglutinins Sodium Nanoparticle chemistry.chemical_element Metal Nanoparticles 02 engineering and technology medicine.disease_cause 01 natural sciences Biochemistry Silver nanoparticle Analytical Chemistry Lectins medicine Escherichia coli Environmental Chemistry Spectroscopy Chromatography biology 010401 analytical chemistry Lens culinaris agglutinin 021001 nanoscience & nanotechnology biology.organism_classification Wheat germ agglutinin 0104 chemical sciences chemistry Plant Lectins 0210 nano-technology Bacteria |
| Zdroj: | Analytica chimica acta. 981 |
| ISSN: | 1873-4324 |
| Popis: | Efficacy of anisotropic silver nanoparticles sensitized with wheat germ agglutinin (WGA) and Lens culinaris agglutinin (LCA) was studied for detection of Staphylococcus aureus and Escherichia coli . It was demonstrated that interaction of these nanoparticles with bacteria stabilizes them and prevents their aggregation upon addition of sodium chloride; such stabilization depends on bacteria concentration. High concentration of bacteria results in higher stabilization whereas low concentration leads to aggregation of nanoparticles. Optical changes as a result of aggregation correlate with bacteria concentration. The developed approach allows the detection of Gram-positive bacteria ( S.aureus ) with the lowest detectable concentration of 10 3 cells/mL and Gram-negative bacteria ( E. coli ) with the lowest detectable concentration of 3 × 10 3 cells/mL using WGA-sensitized nanoparticles. In the case of LCA-sensitized nanoparticles the lowest detection was 5 × 10 3 cells/mL for S. aureus and 5 × 10 4 cells/mL for E. coli . |
| Databáze: | OpenAIRE |
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