Pyridine and p-Nitrophenyl Oxime Esters with Possible Photochemotherapeutic Activity: Synthesis, DNA Photocleavage and DNA Binding Studies
Autor: | Konstantinos Dafnopoulos, Konstantina C. Fylaktakidou, Maria Koffa, Panagiotis S. Gritzapis, Nicolaos-Panagiotis Andreou, Milena Pasolli, Alexandros E. Koumbis, George Psomas |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
amidoxime
oxime esters Pyridines Intercalation (chemistry) Pharmaceutical Science Conjugated system 010402 general chemistry Photochemistry 01 natural sciences ketoxime Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Ultraviolet visible spectroscopy lcsh:Organic chemistry Ethidium aldoxime Oximes Drug Discovery Pyridine DNA photo-cleavers DNA Cleavage Physical and Theoretical Chemistry DNA binding Quenching (fluorescence) Viscosity 010405 organic chemistry Spectrum Analysis Organic Chemistry Esters DNA photo-cleavage Oxime Combinatorial chemistry 0104 chemical sciences chemistry Chemistry (miscellaneous) Molecular Medicine Ethidium bromide |
Zdroj: | Molecules, Vol 21, Iss 7, p 864 (2016) Molecules Molecules; Volume 21; Issue 7; Pages: 864 |
ISSN: | 1420-3049 |
Popis: | Compared to standard treatments for various diseases, photochemotherapy and photo-dynamic therapy are less invasive approaches, in which DNA photocleavers represent promising tools for novel “on demand” chemotherapeutics. A series of p-nitrobenzoyl and p-pyridoyl ester conjugated aldoximes, amidoximes and ethanone oximes were subjected to UV irradiation at 312 nm with supercoiled circular plasmid DNA. The compounds which possessed appropriate properties were additionally subjected to UVA irradiation at 365 nm. The ability of most of the compounds to photocleave DNA was high at 312 nm, whereas higher concentrations were required at 365 nm as a result of their lower UV absorption. The affinity of selected compounds to calf-thymus (CT) DNA was studied by UV spectroscopy, viscosity experiments and competitive studies with ethidium bromide (EB) revealing that all compounds interacted with CT DNA. The fluorescence emission spectra of the pre-treated EB-DNA exhibited a moderate to significant quenching in the presence of the compounds indicating the binding of the compounds to CT DNA via intercalation as concluded also by DNA-viscosity experiments. For the oxime esters the DNA photocleavage and affinity studies aimed to clarify the role of the oxime nature (aldoxime, ketoxime, amidoxime) and the role of the pyridine and p-nitrophenyl moieties both as oxime substituents and ester conjugates. |
Databáze: | OpenAIRE |
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