Immunological characterization of respiratory syncytial virus N protein epitopes recognized by human cytotoxic T lymphocytes
| Autor: | Giuseppa Di Genova, Gianni Gori Savellini, Chiara Terrosi, Patrizia Blardi, Maria Grazia Cusi, Pierpaolo Correale |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Chromium Adolescent Immunology Epitopes T-Lymphocyte chemical and pharmacologic phenomena Biology Lymphocyte Activation Peripheral blood mononuclear cell Virus Epitope Interferon-gamma Virology Cytotoxic T cell Humans Secretion Cells Cultured HLA-A Antigens hemic and immune systems Middle Aged Nucleocapsid Proteins Cytotoxicity Tests Immunologic In vitro Respiratory Syncytial Viruses Cytolysis CTL Leukocytes Mononuclear Molecular Medicine T-Lymphocytes Cytotoxic |
| Zdroj: | Viral immunology. 20(3) |
| ISSN: | 0882-8245 |
| Popis: | Virus-specific cytotoxic T lymphocytes (CTLs) are crucial for the control of respiratory syncytial virus (RSV) infection. This study has identified CTL epitopes of the RSV N protein in healthy subjects. We screened the primary structure of the N protein for HLA-A 0201-binding amino acid consensus motifs, identifying three peptides designated as N-RSV1, N-RSV2, and N-RSV3. These peptides were used to generate CTL lines by stimulating human HLA-A 02.01 peripheral blood mononuclear cells (PBMCs) in vitro. These CTL lines were then characterized by performing CTL chromium release assays and IFN-gamma secretion detection by intracellular cytokine staining. N-RSV1 and N-RSV3 peptides elicited the strongest cytolytic activity against RSV-infected cells and they could be useful epitopes for the analysis of CTL responses to RSV and for understanding immune-induced disease pathogenesis. |
| Databáze: | OpenAIRE |
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