Vibrio coralliirubri sp. nov., a new species isolated from mucus of red coral (Corallium rubrum) collected at Procida island, Italy
Autor: | Kar-Wai Hong, Kok-Gan Chan, Lorena Buono, Ida Romano, Barbara Nicolaus, Javier Pascual, Luigi Leone, Annarita Poli, Pierangelo Orlando, Vincenza Mastascusa, Kian Mau Goh |
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Rok vydání: | 2018 |
Předmět: |
DNA
Bacterial 0301 basic medicine 030106 microbiology red coral DNA Ribosomal Microbiology 03 medical and health sciences Bacterial Proteins RNA Ribosomal 16S Procida island Animals Molecular Biology Phylogeny Vibrio Base Composition Oxidase test Phylogenetic tree Strain (chemistry) biology Fatty Acids General Medicine Anthozoa 16S ribosomal RNA biology.organism_classification Halophile Bacterial Typing Techniques Housekeeping gene Mucus 030104 developmental biology Italy Bacteria |
Zdroj: | Antonie van Leeuwenhoek (Dordr., Online) (2018). doi:10.1007/s10482-017-1013-5 info:cnr-pdr/source/autori:Poli, Annarita; Romano, Ida; Mastascusa, Vincenza; Buono, Lorena; Orlando, Pierangelo; Nicolaus, Barbara; Leone, Luigi; Hong, Kar Wai; Chan, Kok-Gan; Goh, Kian Mau; Pascual, Javier/titolo:Vibrio coralliirubri sp. nov., a new species isolated from mucus of red coral (Corallium rubrum) collected at Procida island, Italy./doi:10.1007%2Fs10482-017-1013-5/rivista:Antonie van Leeuwenhoek (Dordr., Online)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume |
ISSN: | 1572-9699 0003-6072 |
DOI: | 10.1007/s10482-017-1013-5 |
Popis: | Strain Corallo1T was isolated from mucus of red coral (Corallium rubrum) at Punta Pizzaco (Procida island, Naples, Italy). It was characterised as a Gram-stain negative, motile, rod-shaped bacterium. Strain Corallo1T was found to show positive responses for cytochrome-c oxidase, catalase, reduction of nitrate and nitrite, β-galactosidase activity and hydrolysis of starch, xylan, peptone, Tween 40, Tween 80 and casein. Strain Corallo1T was found to be mesophilic, neutrophilic to alkalophilic and slightly halophilic. According to analysis of the almost-complete 16S rRNA gene, strain Corallo1T is closely related to Vibrio celticus (100% sequence similarity), Vibrio gigantis (100%), Vibrio crassostreae (99.7%), Vibrio artabrorum (99.7%) and Vibrio pomeroyi (99.6%). MLSA of five housekeeping genes (atpA, pyrH, recA, rpoA and rpoD) was performed to refine the phylogenetic relationships of strain Corallo1T. A draft genome sequence of strain Corallo1T was obtained. The DNA G+C content of this strain was determined to be 44.5 mol %. The major cellular fatty acids of strain Corallo1T are C16:1, n-C16:0 and C18:1, and the major isoprenoid ubiquinone is Q8. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain Corallo1T represents an independent genomospecies. Based on a polyphasic taxonomic characterisation, strain Corallo1T is concluded to represent a novel species of the genus Vibrio, for which the name Vibrio coralliirubri sp. nov. is proposed. The type strain is Corallo1T (= DSM 27495T = CIP 110630T). |
Databáze: | OpenAIRE |
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