A novel photoacoustic-fluorescent contrast agent for quantitative imaging of lymphatic drainage
| Autor: | J. Carl Kumaradas, Kirsten Cardinell, Xun Zhou, Yeni H. Yücel, Neeru Gupta, Paola Luciani, Hyacinth Irving, Bryan D. Koivisto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Limit of detection lcsh:QC221-246 610 Medicine & health 02 engineering and technology Fluorescence imaging 03 medical and health sciences chemistry.chemical_compound Near-infrared In vivo Lymphatic imaging 540 Chemistry lcsh:QC350-467 Radiology Nuclear Medicine and imaging Minimum detectable concentration 030304 developmental biology 0303 health sciences Bioconjugation Photobleaching Near-infrared spectroscopy 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics lcsh:QC1-999 Contrast agent chemistry Linear range Photostability lcsh:Acoustics. Sound Biophysics 570 Life sciences biology BODIPY 0210 nano-technology lcsh:Physics lcsh:Optics. Light Research Article |
| Zdroj: | Photoacoustics, Vol 21, Iss, Pp 100239-(2021) Cardinell, Kirsten; Gupta, Neeru; Koivisto, Bryan D.; Kumaradas, J. Carl; Zhou, Xun; Irving, Hyacinth; Luciani, Paola; Yücel, Yeni H. (2021). A novel photoacoustic-fluorescent contrast agent for quantitative imaging of lymphatic drainage. Photoacoustics, 21, p. 100239. Elsevier 10.1016/j.pacs.2021.100239 Photoacoustics |
| ISSN: | 2213-5979 |
| Popis: | In vivo near-infrared (NIR) photoacoustic imaging (PAI) studies using novel contrast agents require validation, often via fluorescence imaging. Bioconjugation of NIR dyes to proteins is a versatile platform to obtain contrast agents for specific biomedical applications. Nonfluorescent NIR dyes with higher photostability present advantages for quantitative PAI, compared to most fluorescent NIR dyes. However, they don't provide a fluorescence signal required for fluorescence imaging. Here, we designed a hybrid PA-fluorescent contrast agent by conjugating albumin with a NIR nonfluorescent dye (QC-1) and a visible spectrum fluorescent dye, a BODIPY derivative. The new hybrid tracer QC-1/BSA/BODIPY (QBB) had a low minimum detectable concentration (2.5 mu M), a steep linear range (2.4-54.4 mu M; slope 3.39 E -5), and high photostability. Tracer signal was measured in vivo using PAI to quantify its drainage from eye to the neck and its localization in the neck lymph node was validated with postmortem fluorescence imaging. |
| Databáze: | OpenAIRE |
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