Genetic interaction between glyoxylate pathway regulator UCC1 and La‐motif‐encoding SRO9 regulates stress response and growth rate improvement in Saccharomyces cerevisiae
| Autor: | Shreya Wazir, Aayushi Rakewal, Heena Shoket, Prabhat Kumar, Monika Pandita, Rakesh Kumar, Narendra K Bairwa |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Health Toxicology and Mutagenesis Saccharomyces cerevisiae Glyoxylate cycle petite mutation Toxicology Biochemistry 03 medical and health sciences 0302 clinical medicine RNA Messenger Molecular Biology Messenger RNA 030102 biochemistry & molecular biology biology Chemistry Microfilament Proteins Glyoxylates RNA-Binding Proteins RNA RNA Fungal General Medicine Metabolism biology.organism_classification Cell biology Ubiquitin ligase 030220 oncology & carcinogenesis biology.protein Molecular Medicine Biogenesis |
| Zdroj: | Journal of Biochemical and Molecular Toxicology. 35 |
| ISSN: | 1099-0461 1095-6670 |
| DOI: | 10.1002/jbt.22781 |
| Popis: | Nonavailability of glucose as a carbon source results in glyoxylate pathway activation, which metabolizes nonfermentable carbon for energy generation in Saccharomyces cerevisiae. Ucc1p of S. cerevisiae inhibits activation of the glyoxylate pathway by targeting Cit2p, a key glyoxylate enzyme for ubiquitin-mediated proteasomal degradation when glucose is available as a carbon source. Sro9p, a La-motif protein involved in RNA biogenesis, interacts physically with the messenger RNA of UCC1; however, its functional relevance is yet to be discovered. This study presents binary epistatic interaction between UCC1 and SRO9, with functional implication on the growth rate, response to genotoxic stress, resistance to apoptosis, and petite mutation. Cells with ucc1Δsro9Δ, as their genetic background, exhibit alteration in morphology, improvement in growth rate, resistance to apoptosis, and petite mutation. Moreover, the study indicates a cross-link between ubiquitin-proteasome system and RNA biogenesis and metabolism, with applications in industrial fermentation and screening for cancer therapeutics. |
| Databáze: | OpenAIRE |
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