Immunochromatographic Assay Based on Polydopamine-Decorated Iridium Oxide Nanoparticles for the Rapid Detection of Salbutamol in Food Samples
| Autor: | Kairong Yang, Zhihao Xu, Shuang Zhao, Kunyi He, Tong Bu, Li Wang, Feier Bai, Lihua Li |
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| Rok vydání: | 2021 |
| Předmět: |
Analyte
Indoles Materials science Biocompatibility Polymers Swine education Metal Nanoparticles Nanoparticle Food Contamination Biosensing Techniques 02 engineering and technology Iridium 01 natural sciences Chromatography Affinity Adsorption Limit of Detection parasitic diseases medicine Animals Albuterol General Materials Science Adrenergic beta-2 Receptor Agonists Detection limit Chromatography 010401 analytical chemistry Antibodies Monoclonal 021001 nanoscience & nanotechnology 0104 chemical sciences Red Meat Liver Colloidal gold Pork Meat Salbutamol Cattle Gold 0210 nano-technology Antibodies Immobilized Biosensor medicine.drug |
| Zdroj: | ACS Applied Materials & Interfaces. 13:28899-28907 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Salbutamol (SAL), a β-2 adrenoreceptor agonist, is an unpopular addition to livestock and poultry, causing several side effects to human health. Thus, it is very important to develop a simple and rapid analytical method to screen SAL in the field of food safety. Here, we present an immunochromatographic assay (ICA) method for sensitively detecting SAL with polydopamine-decorated iridium oxide nanoparticles (IrO2@PDA NPs) as a signal tag. The IrO2@PDA with excellent hydrophilicity, biocompatibility, and stability was synthesized by oxidating self-polymerization of dopamine hydrochloride (DAH) on the surface of IrO2 NPs and used to label monoclonal antibodies (mAbs) through simple physical adsorption. Compared with IrO2 NPs, the IrO2@PDA also possessed superior optical properties and higher affinity with mAbs. With the proposed method, the limit of detection for SAL was 0.002 ng/mL, which was improved at least 24-fold and 180-fold compared with the IrO2 NPs-based ICA and conventional gold nanoparticles-based ICA, respectively. Furthermore, the SAL residuals in pork, pork liver, and beef were successfully detected by the developed biosensor and the recoveries ranged from 85.56% to 115.56%. Briefly, this work indicated that the powerful IrO2@PDA-based ICA can significantly improve detection sensitivity and has huge potential for accurate and sensitive detection of harmful small molecules analytes in food safety fields. |
| Databáze: | OpenAIRE |
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