Microdetermination of the prostaglandin B1 in human plasma by gas chromatography/selected ion monitoring using [18O]prostaglandin B1 as an internal standard
| Autor: | Hironori Nakamura, Masataka Ishibashi, Michinao Mizugaki, Yoshiharu Ohyama, Takanori Hishinuma, Kitae Itoh |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Chromatography Gas Prostaglandins B Chromatography Aspirin Microchemistry Metabolite Prostaglandin Ether Derivative Oxygen Isotopes Mass spectrometry Biochemistry Mass Spectrometry Hydrolysis chemistry.chemical_compound Endocrinology chemistry Reference Values Humans Selected ion monitoring Gas chromatography |
| Zdroj: | Prostaglandins. 49:239-246 |
| ISSN: | 0090-6980 |
| DOI: | 10.1016/0090-6980(95)00016-4 |
| Popis: | We devised a simple and effective purification for the microdetermination of prostaglandin B1 (PGB1), a metabolite of PGE1. [18O]PGB1 was synthesized by the repeated base-catalyzed hydrolysis of methyl ester derivatives in H(2)18O, to obtain an internal standard for the gas chromatography/selected ion monitoring (GC/SIM) of PGB1. The methyl ester-methoxime-dimethylisopropylsilyl ether derivative was prepared, then GC/SIM was carried out by monitoring the ion at m/z 448.3 for PGB1 and that at m/z 452.3 for internal standard. A good linear response over the range of 10 pg to 100 ng was demonstrated. We detected PGB1 to a level of about 40 pg/mL in human plasma. This method can be used to determine PGB1 in biological samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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