Genetic variations in NALP1 MRNA expressions in human vitiligo
| Autor: | Ameya R Bhagat, Rajnikant N Shah, Sudha S Deo |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
vitiligo
Autoimmune disease Pathology medicine.medical_specialty integumentary system business.industry NALP1 gene and RT-PCR Dermatology Disease Vitiligo lcsh:RL1-803 medicine.disease medicine.disease_cause Autoimmunity Chromosome 17 (human) Basic Research Depigmentation Immunology Gene expression lcsh:Dermatology Medicine GAPDH Gene medicine.symptom business |
| Zdroj: | Indian Journal of Dermatology, Vol 56, Iss 3, Pp 266-271 (2011) Indian Journal of Dermatology |
| ISSN: | 0019-5154 |
| DOI: | 10.4103/0019-5154.82478 |
| Popis: | Introduction: Vitiligo is an acquired autoimmune disease of unknown etiology showing depigmentation of the skin due to the absence of melanocytes. Familial vitiligo suggests a genetic origin to this disease. Chromosome 17 was recently demonstrated to harbor the gene coding for NALP1. Patients and Methods: A total of 18 patients of vitiligo were selected on the basis of clinical history. Group 1 (N=8) showing segmental or localized vitiligo with one or two macules on the body. Group 2 (N=10) with generalized or whole body vitiligo. A control group of 10 healthy individuals were selected from our laboratory persons with no history or any infections or skin disease. NALP1 gene expression was studied using RT-PCR assay and the bands quantitated as intensity using volume as measurement and comparison of results was done using SPSS 16 version for statistical analysis. NALP1 gene expression was observed in vitiligo patients with different intensities. Results: Greater reduction in the intensity was seen in Group I, which was inversely proportional to the volume of the band. The intensity of the NALP1 and the GAPDH gene expression was more in Group 2 patients than that shown by Group 1. Conclusion: This study shows expression of NALP1 gene in patients as well as normals. NALP1 is widely expressed at low levels but is expressed at high levels in immune cells, particularly T cells and Langerhans cells, in which different patterns are seen that are consistent with the particular involvement of NALP1 in skin autoimmunity. |
| Databáze: | OpenAIRE |
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