Recognition of polyadenosine RNA by zinc finger proteins
| Autor: | Keith M. Berland, Peng Guo, Suzette A. Pabit, Chad M. Kitchen, Kavita A. Marfatia, T. J. Murphy, Anita H. Corbett, Seth M. Kelly |
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| Rok vydání: | 2007 |
| Předmět: |
Riboswitch
Nucleocytoplasmic Transport Proteins Adenosine Saccharomyces cerevisiae Proteins Polymers Molecular Sequence Data RNA-binding protein Saccharomyces cerevisiae Biology Poly(A)-Binding Proteins SR protein Poly(A)-binding protein Humans Signal recognition particle RNA Amino Acid Sequence Conserved Sequence Multidisciplinary Intron RNA Nuclear Proteins RNA-Binding Proteins Zinc Fingers Biological Sciences Non-coding RNA Biochemistry biology.protein Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 104(30) |
| ISSN: | 0027-8424 |
| Popis: | Messenger RNA transcripts are coated from cap to tail with a dynamic combination of RNA binding proteins that process, package, and ultimately regulate the fate of mature transcripts. One class of RNA binding proteins essential for multiple aspects of mRNA metabolism consists of the poly(A) binding proteins. Previous studies have concentrated on the canonical RNA recognition motif-containing poly(A) binding proteins as the sole family of poly(A)-specific RNA binding proteins. In this study, we present evidence for a previously uncharacterized poly(A) recognition motif consisting of tandem CCCH zinc fingers. We have probed the nucleic acid binding properties of a yeast protein, Nab2, that contains this zinc finger motif. Results of this study reveal that the seven tandem CCCH zinc fingers of Nab2 specifically bind to polyadenosine RNA with high affinity. Furthermore, we demonstrate that a human protein, ZC3H14, which contains CCCH zinc fingers homologous to those found in Nab2, also specifically binds polyadenosine RNA. Thus, we propose that these proteins are members of an evolutionarily conserved family of poly(A) RNA binding proteins that recognize poly(A) RNA through a fundamentally different mechanism than previously characterized RNA recognition motif-containing poly(A) binding proteins. |
| Databáze: | OpenAIRE |
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