In vitro susceptibilities of Bartonella and Rickettsia spp. to fluoroquinolone antibiotics as determined by immunofluorescent antibody analysis of infected Vero cell monolayers
| Autor: | Russell L. Regnery, Timothy J. Ives, Eric L. Marston, John D. Butts |
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| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Bartonella Colony Count Microbial Fluorescent Antibody Technique Microbial Sensitivity Tests Microbiology Anti-Infective Agents Levofloxacin Chlorocebus aethiops medicine Animals Pharmacology (medical) Rickettsia Vero Cells Antibacterial agent biology General Medicine bacterial infections and mycoses biology.organism_classification Virology Bartonella elizabethae Ciprofloxacin Infectious Diseases Sparfloxacin Vero cell bacteria Ofloxacin Fluoroquinolones medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents. 18:217-222 |
| ISSN: | 0924-8579 |
| Popis: | The in vitro susceptibilities of Bartonella and Rickettsia spp. to different concentrations of ciprofloxacin, levofloxacin, ofloxacin and sparfloxacin in Vero cell cultures, were determined by enumeration of immunofluorescent-stained bacilli. After incubation in a CO 2 -enriched atmosphere, inocula were replaced and tested with media containing 12 different concentrations of each antibiotic in replicate for each species and the monolayers were re-incubated. Growth status was determined by evaluation of immunofluorescent staining bacilli. Effective inhibitory antibiotic dilution endpoints were determined by counting Bartonella- and Rickettsia-specific fluorescent foci across a range of antibiotic dilutions with an epi-fluorescent microscope, and were compared with an antibiotic-negative control. Based upon the use of C max :MIC and AUC:MIC data, levofloxacin exhibited activity against Bartonella elizabethae and B. quintana . |
| Databáze: | OpenAIRE |
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