A small non-coding RNA facilitates Brucella melitensis intracellular survival by regulating the expression of virulence factor
| Autor: | Wei Zhang, Sun Tao, Cuijuan Duan, Jing Zhang, Guo Xiaojin, Song Lijie, Yiwen Zhao, Xiaoli Yang, Qinfang Hao, Yufei Wang, Zeliang Chen, Yuehua Ke, Xueping Ma, Zhijun Zhong |
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| Rok vydání: | 2018 |
| Předmět: |
Microbiology (medical)
Virulence Factors Virulence Brucella Microbiology Virulence factor Brucellosis 03 medical and health sciences Stress Physiological Brucella melitensis Animals RNA Messenger Gene 030304 developmental biology 0303 health sciences Mice Inbred BALB C Microbial Viability biology 030306 microbiology Intracellular parasite Macrophages General Medicine Gene Expression Regulation Bacterial biology.organism_classification Non-coding RNA RNA Bacterial Infectious Diseases Mutation RNA Small Untranslated Female Intracellular Spleen |
| Zdroj: | International journal of medical microbiology : IJMM. 309(3-4) |
| ISSN: | 1618-0607 |
| Popis: | Brucella species are the causative agents of brucellosis, a worldwide zoonotic disease that affects a broad range of mammals and causes great economic losses. Small regulatory RNAs (sRNAs) are post-transcriptional regulatory molecules that participate in the stress adaptation and pathogenesis of Brucella. In this study, we characterized the role of a novel sRNA, BSR1141, in the intracellular survival and virulence of Brucella melitensis. The results show that BSR1141 was highly induced during host infections and under in vitro stress situations that simulated the conditions encountered within host phagocytes. In addition, a BSR1141 mutant showed reduced survival both under in vitro stress conditions and in mice, confirming the role of BSR1141 in Brucella intracellular survival. Bioinformatic and experimental approaches revealed that BSR1141 affects the expression of many target genes, including the Brucella virulence component virB2. These data indicate that BSR1141 could influence the expression of virB2, which is important for B. melitensis pathogenesis and intracellular survival. This work provides new insight into the mechanism of adaptation to environmental stress and into the pathogenesis of intracellular pathogens. |
| Databáze: | OpenAIRE |
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