Immunostimulatory effects of platinum compounds: correlation between sensitizing properties in vivo and modulation of receptor-mediated endocytosis in vitro
| Autor: | Hans-Christian Schuppe, Jürgen Knop, Johanna Kulig, Peter Kind, Ulrich Kühn, Uwe Lempertz, Detief Becker |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Hypersensitivity
Immediate Langerhans cell Lymphocyte T-Lymphocytes Immunology Platinum Compounds Biology Immunologic Tests In Vitro Techniques Endocytosis Lymphocyte Activation Mice Immune system Adjuvants Immunologic Chlorides In vivo medicine Immunology and Allergy Animals Humans Mice Inbred BALB C Cell growth General Medicine Receptor-mediated endocytosis In vitro medicine.anatomical_structure Dermatitis Occupational Lymph Nodes Cisplatin |
| Zdroj: | International archives of allergy and immunology. 112(2) |
| ISSN: | 1018-2438 |
| Popis: | The sensitizing properties of different complex salts of platinum were defined in vivo by means of the popliteal lymph node (PLN) assay in mice. Hexa- and tetrachloroplatinates were confirmed to be highly immunogenic, inducing vigorous primary immune responses in the draining PLN following single subcutaneous injections. Flow-cytometric analysis revealed a dramatic increase in the total number of cells expressing proliferating cell nuclear antigen. The majority of these cells were of the T helper phenotype (CD4+) reflecting the T-cell dependence of the PLN response induced by Pt salts such as Na2[PtCl6] or Na2[PtCl4]. In contrast, [Pt(NH3)4]Cl2 failed to elicit a significant increase in PLN cell proliferation when compared with saline-treated controls. The differential immunogenicity of the Pt compounds found in vivo directly correlated with their capacity to modulate mechanisms of receptor-mediated endocytosis in murine Langerhans cells in vitro. The reactivity of Na2[PtCl6] or Na2[PtCl4] resembled that of potent contact sensitizers in this endocytosis assay whereas [Pt(NH3)4]Cl2 proved to be inert. These results suggest that [Pt(NH3)4]Cl2 might be less harmful to humans than hexa- or tetrachloroplatinates. As demonstrated with Pt compounds, monitoring of direct effects of low-molecular-weight chemicals on antigen-presenting dendritic cells in vitro is able to predict their sensitizing potential in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|