Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice
| Autor: | Norah G. Verbout, Andrew J. Halayko, David I. Kasahara, Huiqing Si, Allison P. Wurmbrand, Christopher Hug, Stephanie A. Shore, Alison S. Williams, Leandro A.P. Benedito |
|---|---|
| Přispěvatelé: | University of Manitoba |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Article Subject Transgene Inflammation 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Immunology and Allergy Sensitization 030304 developmental biology Basement membrane 0303 health sciences biology Adiponectin medicine.diagnostic_test business.industry respiratory system 3. Good health Ovalbumin Endocrinology medicine.anatomical_structure Bronchoalveolar lavage 030228 respiratory system biology.protein medicine.symptom business Research Article Hormone |
| Zdroj: | Journal of Allergy |
| ISSN: | 1687-9783 |
| DOI: | 10.1155/2013/349520 |
| Popis: | Obesity is an important risk factor for asthma. Obese individuals have decreased circulating adiponectin, an adipose-derived hormone with anti-inflammatory properties. We hypothesized that transgenic overexpression of adiponectin would attenuate allergic airways inflammation and mucous hyperplasia in mice. To test this hypothesis, we used mice overexpressing adiponectin (Adipo Tg). Adipo Tg mice had marked increases in both serum adiponectin and bronchoalveolar lavage (BAL) fluid adiponectin. Both acute and chronic ovalbumin (OVA) sensitization and challenge protocols were used. In both protocols, OVA-induced increases in total BAL cells were attenuated in Adipo Tg versus WT mice. In the acute protocol, OVA-induced increases in several IL-13 dependent genes were attenuated in Adipo Tg versus WT mice, even though IL-13 per se was not affected. With chronic exposure, though OVA-induced increases in goblet cells numbers per millimeter of basement membrane were greater in Adipo Tg versus WT mice, mRNA abundance of mucous genes in lungs was not different. Also, adiponectin overexpression did not induce M2 polarization in alveolar macrophages. Our results indicate that adiponectin protects against allergen-induced inflammatory cell recruitment to the airspaces, but not development of goblet cell hyperplasia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|