Tobamoviruses of two new species trigger resistance in pepper plants harbouring functional L alleles
| Autor: | Tatsuya Nagata, Jefferson B. Vélez-Olmedo, Fernando L. Melo, Renato O. Resende, Athos Silva de Oliveira, C. E. Fribourg |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pepper mild mottle virus Genome Viral 01 natural sciences Genome Host Specificity 03 medical and health sciences Virology Pepper medicine DNA Primers Plant Diseases Base Sequence biology Host (biology) Inoculation Tobamovirus fungi food and beverages biology.organism_classification medicine.disease Indicator plant 030104 developmental biology DNA Viral Mottle Capsicum 010606 plant biology & botany |
| Zdroj: | Journal of General Virology. 102 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/jgv.0.001524 |
| Popis: | Tobamoviruses are often referred to as the most notorious viral pathogens of pepper crops. These viruses are not transmitted by invertebrate vectors, but rather by physical contact and seeds. In this study, pepper plants displaying mild mottle and mosaic symptoms were sampled in four different regions of Peru. Upon double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) tests, seven samples cross-reacted weakly with antibodies against pepper mild mottle virus (PMMoV), suggesting the presence of tobamoviruses. When employing RT-PCR, conserved primers amplified cDNA fragments of viruses from two putative new tobamovirus species in the samples. The complete genome of two representative isolates were, therefore, sequenced and analysed in silico. These viruses, which were tentatively named yellow pepper mild mottle virus (YPMMoV) and chilli pepper mild mottle virus (CPMMoV), shared highest nucleotide genome sequence identities of 83 and 85 % with bell pepper mottle virus (BpeMV), respectively. Mechanical inoculation of indicator plants with YPMMoV and CPMMoV isolates did not show any obvious differences in host ranges. These viruses were also inoculated mechanically on pepper plants harbouring different resistance L alleles to determine their pathotypes. Pepper plants carrying unfunctional L alleles (L 0) to tobamoviruses were infected by all isolates and presented differential symptomatology for YPMMoV and CPMMoV. On the other hand, pepper plants carrying L 1, L 2, L 3 and L 4 alleles were resistant to all isolates, indicating that these viruses belong to pathotype P0. |
| Databáze: | OpenAIRE |
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