Comparison of single euploid blastocyst transfer cycle outcome derived from embryos with normal or abnormal cleavage patterns
| Autor: | Lale Karakoc Sokmensuer, Hakan Yarali, İrem Y Özbek, Mehtap Polat, Gurkan Bozdag, Sezcan Mumusoglu |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
0301 basic medicine Embryonic Development Aneuploidy Lower priority Biology Cleavage (embryo) Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy medicine Humans Blastocyst reproductive and urinary physiology Retrospective Studies 030219 obstetrics & reproductive medicine Blastocyst Transfer Outcome measures Obstetrics and Gynecology Embryo Embryo Transfer Embryo Mammalian medicine.disease 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Female Ploidy Live Birth Developmental Biology |
| Zdroj: | Reproductive BioMedicine Online. 42:892-900 |
| ISSN: | 1472-6483 |
| DOI: | 10.1016/j.rbmo.2021.02.005 |
| Popis: | To assess incidence of abnormal cleavage among biopsied blastocysts; to compare euploidy rates of the blastocysts with abnormal and normal cleavage; and to compare single euploid blastocyst transfer (SEBT) outcome derived from embryos with normal or abnormal cleavage.Retrospective analysis of prospectively collected data in a private IVF clinic. Consecutive 554 patients (749 cycles) undergoing preimplantation genetic testing for aneuploidy (n = 497; 671 cycles) or monogenic diseases (n = 57; 78 cycles) were included. All assessments for abnormal cleavage were carried out retrospectively; presence of abnormal cleavage was not a factor in deciding which euploid embryo to transfer. A total of 1015 blastocysts were biopsied and 295 SEBT procedures were carried out. Main outcome measure was live birth rate (LBR).Incidence of reverse cleavage, direct cleavage, and reverse plus direct cleavage, were 7.7%, 6.4% and 2.3%, respectively. Of the 1015 biopsied blastocysts, 35.0% were euploid. Blastocysts with abnormal cleavage, in total, had a significantly higher euploidy rate compared with blastocysts with normal cleavage (44.6% [74/166] versus 33.1% [281/849]; P = 0.017). The LBR after SEBT with normal, reverse and direct cleavage, and direct cleavage plus reverse cleavage, was 133/238 (55.9%), 6/26 (23.1%), 8/24 (33.3%) and 0/3 (0.0%) (P0.001). Generalized estimating equation analysis showed that the presence of abnormal cleavage pattern was the only independent predictor of LBR (OR 0.316; 95% CI 0.115 to 0.867; P = 0.013).Blastocysts with direct or reverse cleavage should be biopsied in preimplantation genetic testing cycles if they are morphologically eligible. Euploid blastocysts with abnormal cleavage, however, have approximately half the LBR of those euploid blastocyst with normal cleavage, hence, blastocysts with abnormal cleavage should have lower priority for transfer. |
| Databáze: | OpenAIRE |
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