Unraveling altered RNA metabolism in pancreatic cancer cells by liquid-chromatography coupling to ion mobility mass spectrometry

Autor:	Simon Lagies, Johannes Plagge, Thalia Erbes, Uwe A. Wittel, Lukas Braun, Bernd Kammerer, Manuel Schlimpert, Michel Kather
Rok vydání:	2019
Předmět:	Ion-mobility spectrometry 02 engineering and technology Mass spectrometry 01 natural sciences Biochemistry Modified nucleosides Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Pancreatic cancer Cell Line Tumor Ion Mobility Spectrometry medicine Biomarkers Tumor Humans RNA Neoplasm Phenylboronic acid RNA metabolism Chromatography 010401 analytical chemistry Cancer 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Coupling (electronics) Pancreatic Neoplasms chemistry 0210 nano-technology
Zdroj:	Analytical and bioanalytical chemistry. 411(24)
ISSN:	1618-2650
Popis:	Ion mobility coupling to mass spectrometry facilitates enhanced identification certitude. Further coupling to liquid chromatography results in multi-dimensional analytical methods, especially suitable for complex matrices with structurally similar compounds. Modified nucleosides represent a large group of very similar members linked to aberrant proliferation. Besides basal production under physiological conditions, they are increasingly excreted by transformed cells and subsequently discussed as putative biomarkers for various cancer types. Here, we report a method for modified nucleosides covering 37 species. We determined collisional cross-sections with high reproducibility from pure analytical standards. For sample purification, we applied an optimized phenylboronic acid solid-phase extraction on media obtained from four different pancreatic cancer cell lines. Our analysis could discriminate different subtypes of pancreatic cancer cell lines. Importantly, they could clearly be separated from a pancreatic control cell line as well as blank medium. m1A, m27G, and Asm were the most important features discriminating cancer cell lines derived from well-differentiated and poorly differentiated cancers. Eventually, we suggest the analytical method reported here for future tumor-marker identification studies. Graphical abstract.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee9aebed22aac3620fe50b05b3695955 https://pubmed.ncbi.nlm.nih.gov/31037374 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.