Substance P immunoreactivity of rat brain stem neurons in primary culture
| Autor: | M. Denavit-Saubie, Thierry Didier Jacquin, J. P. Denizot |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Phosphatase
Central nervous system Immunocytochemistry Neuropeptide Cell Count Substance P Biology Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Animals Neurotransmitter Cells Cultured Neurons Cell Differentiation Rats Inbred Strains Molecular biology Rats medicine.anatomical_structure nervous system chemistry Cell culture Immunology Immunohistochemistry Brain Stem |
| Zdroj: | Journal of Neuroscience Research. 31:131-135 |
| ISSN: | 1097-4547 0360-4012 |
| DOI: | 10.1002/jnr.490310118 |
| Popis: | Substance P (SP)-ergic neurons from 16/17 day-embryonic rat brain stem in primary culture were identified by immunocytochemistry using biotinylated avidine and phosphatase alcaline methods with affinity-purified anti-SP antibodies. An average of 84% of neurons contained SP from day 9 to day 21 after plating. These in vitro data show that SP-containing neurons develop in our culture conditions. SP may act as a maturation factor as well as a neurotransmitter. |
| Databáze: | OpenAIRE |
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