Genome-wide identification and analysis of the ALTERNATIVE OXIDASE gene family in diploid and hexaploid wheat
| Autor: | Eric H. Roalson, Zara B. York, Vandhana Krishnan, Karen A. Sanguinet, Rhoda A. T. Brew-Appiah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Protein Structure Comparison
0106 biological sciences 0301 basic medicine lcsh:Medicine Plant Science Hexaploidy Biochemistry 01 natural sciences Genome Database and Informatics Methods Gene Expression Regulation Plant Plant Resistance to Abiotic Stress Macromolecular Structure Analysis lcsh:Science Phylogeny Triticum Plant Proteins Genetics Multidisciplinary Ecology biology Gene Expression Regulation Developmental Eukaryota food and beverages Genomics Plants Multigene Family Plant Physiology Wheat Ploidy Oxidoreductases Sequence Analysis Genome Plant Herbicide Resistance Research Article Protein Structure Alternative oxidase Bioinformatics Aegilops Genes Plant Research and Analysis Methods Genome Complexity Mitochondrial Proteins Polyploidy 03 medical and health sciences Stress Physiological Sequence Motif Analysis Gene Types Plant-Environment Interactions Gene family Aegilops tauschii Plant Defenses Grasses Molecular Biology Gene Whole Genome Sequencing Plant Ecology Ecology and Environmental Sciences lcsh:R Organisms Biology and Life Sciences Computational Biology Proteins Plant Pathology biology.organism_classification Diploidy Introns Aegilops speltoides 030104 developmental biology Triticum urartu Regulator Genes lcsh:Q Departures from Diploidy Sequence Alignment 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 13, Iss 8, p e0201439 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | A comprehensive understanding of wheat responses to environmental stress will contribute to the long-term goal of feeding the planet. ALERNATIVE OXIDASE (AOX) genes encode proteins involved in a bypass of the electron transport chain and are also known to be involved in stress tolerance in multiple species. Here, we report the identification and characterization of the AOX gene family in diploid and hexaploid wheat. Four genes each were found in the diploid ancestors Triticum urartu, and Aegilops tauschii, and three in Aegilops speltoides. In hexaploid wheat (Triticum aestivum), 20 genes were identified, some with multiple splice variants, corresponding to a total of 24 proteins for those with observed transcription and translation. These proteins were classified as AOX1a, AOX1c, AOX1e or AOX1d via phylogenetic analysis. Proteins lacking most or all signature AOX motifs were assigned to putative regulatory roles. Analysis of protein-targeting sequences suggests mixed localization to the mitochondria and other organelles. In comparison to the most studied AOX from Trypanosoma brucei, there were amino acid substitutions at critical functional domains indicating possible role divergence in wheat or grasses in general. In hexaploid wheat, AOX genes were expressed at specific developmental stages as well as in response to both biotic and abiotic stresses such as fungal pathogens, heat and drought. These AOX expression patterns suggest a highly regulated and diverse transcription and expression system. The insights gained provide a framework for the continued and expanded study of AOX genes in wheat for stress tolerance through breeding new varieties, as well as resistance to AOX-targeted herbicides, all of which can ultimately be used synergistically to improve crop yield. |
| Databáze: | OpenAIRE |
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