Bacillus subtilis as a host for mosquitocidal toxins production
| Autor: | Giulia Barbieri, Giuliano Gasperi, Paolo Gabrieli, Viola Camilla Scoffone, Alessandra M. Albertini, Angelica Pellegrini, Emanuela Ursino, Alessandro Di Cosimo, Giulia Fiorentino |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Aedes albopictus
lcsh:Biotechnology Bacillus thuringiensis Bioengineering Bacillus subtilis Mosquito Vectors medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Microbiology 03 medical and health sciences Hemolysin Proteins Bacterial Proteins Aedes lcsh:TP248.13-248.65 Gene expression medicine Animals Pest Control Biological Gene Research Articles 030304 developmental biology 0303 health sciences biology Bacillus thuringiensis Toxins 030306 microbiology Host (biology) Toxin fungi Promoter Bacillus thuringiensis israelensis biology.organism_classification Endotoxins Biotechnology Research Article |
| Zdroj: | Microbial Biotechnology Microbial Biotechnology, Vol 13, Iss 6, Pp 1972-1982 (2020) |
| ISSN: | 1751-7915 |
| Popis: | Summary Aedes albopictus transmits several arboviral infections. In the absence of vaccines, control of mosquito populations is the only strategy to prevent vector‐borne diseases. As part of the search for novel, biological and environmentally friendly strategies for vector control, the isolation of new bacterial species with mosquitocidal activity represents a promising approach. However, new bacterial isolates may be difficult to grow and genetically manipulate. To overcome these limits, here we set up a system allowing the expression of mosquitocidal bacterial toxins in the well‐known genetic background of Bacillus subtilis. As a proof of this concept, the ability of B. subtilis to express individual or combinations of toxins of Bacillus thuringiensis israelensis (Bti) was studied. Different expression systems in which toxin gene expression was driven by IPTG‐inducible, auto‐inducible or toxin gene‐specific promoters were developed. The larvicidal activity of the resulting B. subtilis strains against second‐instar Ae. albopictus larvae allowed studying the activity of individual toxins or the synergistic interaction among Cry and Cyt toxins. The expression systems here presented lay the foundation for a better improved system to be used in the future to characterize the larvicidal activity of toxin genes from new environmental isolates. We investigated the feasibility of using B. subtilis as host to produce known and yet unknown mosquitocidal bacterial toxins. As a proof of this concept, by using different expression systems, the ability of B. subtilis to express individual or combinations of toxins of Bacillus thuringiensis israelensis (Bti) was studied. The toxicity of the obtained recombinant strains against Ae. albopictus larvae was assessed. |
| Databáze: | OpenAIRE |
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