The JNK phosphatase M3/6 is inhibited by protein-damaging stress
| Autor: | Carmen Palacios, Mary Collins, Gordon R. Perkins |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Osmotic shock
Phosphatase General Biochemistry Genetics and Molecular Biology Heating Dephosphorylation 03 medical and health sciences 0302 clinical medicine Dual-specificity phosphatase 030304 developmental biology 0303 health sciences Agricultural and Biological Sciences(all) biology Biochemistry Genetics and Molecular Biology(all) Kinase c-jun JNK Mitogen-Activated Protein Kinases Molecular biology Cell biology Solubility Mitogen-activated protein kinase biology.protein Dual-Specificity Phosphatases Phosphorylation Mitogen-Activated Protein Kinases Protein Tyrosine Phosphatases General Agricultural and Biological Sciences 030217 neurology & neurosurgery |
| Zdroj: | Current Biology. 11:1439-1443 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/s0960-9822(01)00426-2 |
| Popis: | Cells respond to stresses such as osmotic shock and heat shock by activating stress-activated protein kinases (SAPKs), including c-Jun N-terminal kinase (JNK) [1]. Activation of JNK requires phosphorylation of threonine and tyrosine residues in the TPY activation loop motif [2, 3] and can be reversed by the removal of either phosphate group. Numerous JNK phosphatases including dual-specificity phosphatases [4, 5], have been identified. Many stimuli activate JNK by increasing its rate of phosphorylation; however, JNK dephosphorylation is inhibited in cells after heat shock [6], suggesting that a JNK phosphatase(s) is inactivated. M3/6 is a dual-specificity phosphatase selective for JNK [7, 8]. We have previously expressed M3/6 in the mouse bone marrow cell line BAF3 in order to show that JNK activation by IL-3 is necessary for cell survival and proliferation [9]. Here we report that M3/6 dissociates from JNK and appears in an insoluble fraction after heat shock. These data identify M3/6 as a JNK phosphatase that is inactivated by heat shock and provide a molecular mechanism for the activation of JNK by heat shock. |
| Databáze: | OpenAIRE |
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