Reactive oxygen species altering the metabolite profile of the marine-derived fungus Dichotomomyces cejpii F31-1
| Autor: | Wen-Jian Lan, Wen-Zhe Ma, Jun Dong, Sharpkate Shaker, Yong-Wei Guo, Ting-Ting Sun, Liang-Yue Wang, Depo Yang, Long-Ping Zhu, Hou-Jin Li, Yan-Xiu Chen, Dong-Lan Wu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Ergosterol Reactive oxygen species Chromatography biology 010405 organic chemistry Metabolite Organic Chemistry chemistry.chemical_element Plant Science Fungus biology.organism_classification 01 natural sciences Biochemistry High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry 010404 medicinal & biomolecular chemistry chemistry.chemical_compound chemistry Chlorine Hydrogen peroxide Diketopiperazines |
| DOI: | 10.6084/m9.figshare.8293934 |
| Popis: | To investigate the influence of reactive oxygen species (ROS) on the secondary metabolites of the marine-derived fungus Dichotomomyces cejpii F31-1, hydrogen peroxide (H2O2) was added to the GPY culture medium. The HPLC chromatogram of the EtOAc extract of the culture broth was distinct from that of the H2O2 free GPY medium. Further study of the metabolites in the GPY medium with H2O2 resulted in the discovery of eight known compounds. Among them, (22E)-5α, 8α-epidioxyergosta-6, 22-dien-3β-ol (2) and ergosta-4,6,8(14),22-tetraene-3-one (3) were present in the highest concentration, while ergosterol and diketopiperazines are abundant in the H2O2 free medium. Additionally, a new compound, dichocetide D (1) containing a chlorine element and a known ergosterol (10) were isolated from the H2O2 free medium. (22E)-5α, 8α-epidioxyergosta-6, 22-dien-3β-ol (2) exhibited moderate cytotoxic activity against human prostate cancer cell line LNCaP-C4-2B. |
| Databáze: | OpenAIRE |
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