Cell-Based Fabrication of Organic/Inorganic Composite Gel Material
| Autor: | Takuya Matsumoto, Jun Ichi Sasaki, Ami Mizuno, Miki Kashiwagi, Shin Suke Yoshida, Takayoshi Nakano |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Fabrication
Materials science Composite number lcsh:Technology Article medicine General Materials Science lcsh:Microscopy lcsh:QC120-168.85 lcsh:QH201-278.5 lcsh:T Mesenchymal stem cell biomimetic material In vitro hydrogel organic/inorganic composite 3D medicine.anatomical_structure Chemical engineering Cell culture lcsh:TA1-2040 lcsh:Descriptive and experimental mechanics Bone marrow lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 Cell based Biomedical engineering Biomineralization |
| Zdroj: | Materials Materials; Volume 4; Issue 1; Pages: 327-338 Materials, Vol 4, Iss 1, Pp 327-338 (2011) |
| ISSN: | 1996-1944 |
| Popis: | Biomaterials containing components similar to the native biological tissue would have benefits as an implantable scaffold material. To obtain such biomimetic materials, cells may be great contributors because of their crucial roles in synthetic organics. In addition, the synthesized organics—especially those derived from osteogenic differentiated cells—become a place where mineral crystals nucleate and grow even in vitro. Therefore, to fabricate an organic/inorganic composite material, which is similar to the biological osteoid tissue, bone marrow derived mesenchymal stem cells (BMSCs) were cultured in a 3D fibrin gel in this study. BMSCs secreted bone-related proteins that enhanced the biomineralization within the gel when the cells were cultured with an osteogenic differentiation medium. The compositions of both synthesized matrices and precipitated minerals in the obtained materials altered depending on the cell culture period. The mineral obtained in the 3D gel showed low crystalline hydroxyapatite. The composite materials also showed excellent osteoconductivity with new bone formation when implanted in mice tibiae. Thus, we demonstrated the contributions of cells for fabricating implantable organic/inorganic composite gel materials and a method for controlling the material composition in the gel. This cell-based material fabrication method would be a novel method to fabricate organic/inorganic composite biomimetic materials for bone tissue engineering. |
| Databáze: | OpenAIRE |
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