Detection of somatostatin receptor subtype 2 (SSTR2) in established tumors and tumor cell lines: Evidence for SSTR2 heterogeneity
| Autor: | John E. Taylor, Magali Theveniau, Roya Bashirzadeh, Terry Reisine, Peter A. Eden |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Lung Neoplasms Physiology Molecular Sequence Data Gene Expression Breast Neoplasms In Vitro Techniques Biology Polymerase Chain Reaction Biochemistry Mice Cellular and Molecular Neuroscience Endocrinology Neoplasms Gene expression Tumor Cells Cultured Somatostatin receptor 3 Animals Humans Somatostatin receptor 2 Somatostatin receptor 1 RNA Messenger Receptors Somatostatin Receptor Melanoma DNA Primers Gastrointestinal Neoplasms Delta cell Base Sequence Somatostatin receptor Prostatic Neoplasms DNA Neoplasm Molecular biology Rats Kinetics Somatostatin Female |
| Zdroj: | Peptides. 15:1229-1236 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/0196-9781(94)90146-5 |
| Popis: | The somatostatin receptor subtype 2 (SSTR2) was detected in a wide range of human and rat tumors using in vitro receptor binding ([125I]MK-678), receptor gene expression analysis, and immunoblotting techniques. The highest receptor concentrations were observed in the rat AR42J pancreatic and human small cell lung cancer (SCLC) cell lines, NCI-H69 and NCI-H345, with much lower levels detected in breast, prostate, melanoma, and hepatic tumors. Several human pancreas tumors were devoid of SSTR2. For all tumors showing detectable [125I]MK-678 binding, SSTR2 receptor mRNA was expressed. Furthermore, a mRNA transcript corresponding to a truncated isoform of SSTR2 was detected at low levels in the human SCLC NCI-H69 cell line, and likely represents a human homologue of rodent SSTR2B. Immunoblotting analysis using the SSTR2-specific antibody, 2e3, detected multiple immunoreactive protein species, including a predominant 150-kDa molecule, which could be blocked by the SSTR2-derived 2e3 peptide. Somatostatin (SRIF) peptides with high SSTR2 affinity and antiproliferative properties were potent inhibitors of [125I]MK-678 binding to several tumor types, suggesting that they may exert antitumor effects via the SSTR2 receptor. |
| Databáze: | OpenAIRE |
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