Determination of Serum Nucleotidase with Cytidine Monophosphate as Substrate, (I)
| Autor: | W. van der Slik, P. J. van der Kooij, J.-P. Persijn, C. B. Korsten |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Cytidine monophosphate
Chromatography Adenosine Deaminase education Biochemistry (medical) Clinical Biochemistry Deamination Substrate (chemistry) Cytidine General Medicine Cytosine Nucleotides Adenosine Monophosphate Ammonia chemistry.chemical_compound Indophenol chemistry Biochemistry Nucleotidases Spectrophotometry Cytidine Deaminase Nucleotidase Escherichia coli Magnesium |
| Zdroj: | Clinical Chemistry and Laboratory Medicine. 13 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm.1975.13.4.149 |
| Popis: | This paper deals with a new method for the determination of serum nucleotidase (EC 3.1.3.5). The assay is performed with cytidine-5'-monophosphate as substrate, followed by deamination of the generated cytidine. The principle of the method and the determination of the liberated ammonia by the Berthelot indophenol-reaction are comparable to the Persijn--van der Slik method in which adenosine-5'-monophosphate is used as substrate. The correlation between the results obtained with these two methods was found to be good; the new method has the advantage of higher sensitivity. |
| Databáze: | OpenAIRE |
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