A micelle model for the sedimentation behavior of bovine β-casein
| Autor: | Gerson Kegeles, Mei-sheng Tai |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Macromolecular Substances
Protein Conformation Sedimentation (water treatment) Biophysics Mineralogy Thermodynamics Biochemistry Micelle law.invention chemistry.chemical_compound law Animals Colloids Micelles chemistry.chemical_classification Range (particle radiation) Chemistry Organic Chemistry Caseins Polymer Monomer Critical micelle concentration Particle Cattle Electron microscope Ultracentrifugation Mathematics |
| Zdroj: | Biophysical Chemistry. 20:81-87 |
| ISSN: | 0301-4622 |
| DOI: | 10.1016/0301-4622(84)80007-1 |
| Popis: | The monomer-single polymer model of G.A. Gilbert (Disc. Faraday Soc. 20 (1955) 68) for moving boundary sedimentation has been used by Payens and colleagues to explain the observed results for bovine caseins, and by Harrington and colleagues to explain the observed results for myosin fibrils. Electron microscope pictures of Buchheim and Schmidt have subsequently revealed micellar β-casein in the form of slightly elongated or spherical particles having a bimodal size distribution, but with a broad range of particle sizes, at concentrations not too far above the critical micelle concentration. The equilibrium properties of a broadly distributed micellar system can be fitted by the shell model developed by one of us. and in the present article, the shell model is extended to predict the moving boundary sedimentation behavior of such a system. The observed sedimentation patterns, as well as the critical concentration predictions of the monomer-single polymer Gilbert sedimentation model, are satisfactorily described with the present model, based on a continuous distribution of intermediates between monomers and the largest possible spherical micelles. For one example considered, the predicted frequency distribution of molecular weight is in qualitative agreement with the frequency distribution of particle volume found by Buchheim and Schmidt. |
| Databáze: | OpenAIRE |
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