IFITM1, CD10, SMA, and h-caldesmon as a helpful combination in differential diagnosis between endometrial stromal tumor and cellular leiomyoma
| Autor: | Wei Jia, Ruiqi Zhang, Xihua Shen, Hong Zou, Zhenzhu Sun, Xin Xiong, Weilin Zhao, Lijuan Pang, Chun Wang, Xinhua Ji, Mei Cui, Lin Tao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Stromal cell Cellular leiomyoma Sensitivity and Specificity Diagnosis Differential Antigens Neoplasm Endometrial Stromal Tumors Genetics medicine Biomarkers Tumor Humans SMA RC254-282 Aged H-caldesmon Tissue microarray Cluster of differentiation biology Leiomyoma Chemistry Neoplasms. Tumors. Oncology. Including cancer and carcinogens Muscle Smooth Endometrial stromal tumor Middle Aged Antigens Differentiation Immunohistochemistry Actins Endometrial Neoplasms IFITM1 Oncology Area Under Curve Uterine Neoplasms biology.protein Biomarker (medicine) CD10 Calmodulin-Binding Proteins Female Neprilysin Differential diagnosis Antibody |
| Zdroj: | BMC Cancer, Vol 21, Iss 1, Pp 1-9 (2021) |
| ISSN: | 1471-2407 |
| Popis: | Background The differential diagnosis of endometrial stromal tumor (EST) and uterine cellular leiomyoma (CL) remains a challenge in clinical practice, especially low grade endometrial stromal sarcoma (ESS) and CL, suggesting the need for novel immunomarkers panels for differential diagnosis. Interferon-induced transmembrane protein 1 (IFITM1) is a novel immunomarker for endometrial stromal cells, h-caldesmon is an immunomarker for smooth muscle cells and has a higher specificity than smooth muscle actin (SMA). So this study aimed to evaluate whether IFITM1, cluster of differentiation 10(CD10), SMA, and h-caldesmon are useful biomarker combinations for the differential diagnosis of EST and CL. Methods Tissue microarrays were used to detect IFITM1, CD10, SMA, and h-caldesmon immunohistochemical staining in 30 EST and 33 CL cases. Results The expressions of IFITM1 and CD10 were high in EST (86.7 and 63.3%, respectively) but low in CL (18.2 and 21.2%), whereas those of h-caldesmon and SMA were high in CL (87.9 and 100%) and low in EST (6.9 and 40%). In diagnosing EST, IFITM1 shows better sensitivity and specificity (86.7 and 81.8%, respectively) than CD10 (63.3 and 78.8%). The specificity of h-caldesmon in diagnosing CL was significantly higher (93.1%) than that of SMA (60%). When all four antibodies were combined for the differential diagnosis, the area-under-the-curve (AUC) predictive value was 0.995. The best combination for diagnosing EST was IFITM1 (+) or CD10 (+) and h-caldesmon (−) (sensitivity 86.7%, specificity 93.9%). Conclusion The best combination for diagnosing CL were h-caldesmon (+) and SMA (+) (sensitivity 87.9%, specificity 100%). IFITM1, CD10, SMA, and h-caldesmon are a good combination for the differential diagnosis of EST and CL. |
| Databáze: | OpenAIRE |
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