Streptococcus azizii sp. nov., isolated from naïve weanling mice
| Autor: | Patricia L. Shewmaker, Anne M. Whitney, Neil S. Lipman, Christopher A. Gulvik |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial Male 0301 basic medicine Firmicutes medicine.disease_cause Microbiology Genome Mice 03 medical and health sciences Nucleic acid thermodynamics Taxonomic Description Meningoencephalitis Phylogenetics RNA Ribosomal 16S medicine Animals Gene Phylogeny Ecology Evolution Behavior and Systematics Genetics Base Composition biology Streptococcus Nucleic Acid Hybridization Sequence Analysis DNA General Medicine biology.organism_classification rpoB 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Genes Bacterial Female |
| Zdroj: | Int J Syst Evol Microbiol |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.002407 |
| Popis: | Three isolates of a previously reported novel catalase-negative, Gram-stain-positive, coccoid, alpha-haemolytic, Streptococcus species that were associated with meningoencephalitis in naïve weanling mice were further evaluated to confirm their taxonomic status and to determine additional phenotypic and molecular characteristics. Comparative 16S rRNA gene sequence analysis showed nearly identical intra-species sequence similarity (≥99.9 %), and revealed the closest phylogenetically related species, Streptococcus acidominimus and Streptococcuscuniculi, with 97.0 and 97.5 % sequence similarity, respectively. The rpoB, sodA and recN genes were identical for the three isolates and were 87.6, 85.7 and 82.5 % similar to S. acidominimus and 89.7, 86.2 and 80.7 % similar to S. cuniculi , respectively. In silico DNA–DNA hybridization analyses of mouse isolate 12-5202(T) against S. acidominimus CCUG 27296(T) and S. cuniculi CCUG 65085(T) produced estimated values of 26.4 and 25.7 % relatedness, and the calculated average nucleotide identity values were 81.9 and 81.7, respectively. These data confirm the taxonomic status of 12-5202(T) as a distinct Streptococcus species, and we formally propose the type strain, Streptococcus azizii 12-5202(T) (=CCUG 69378(T)=DSM 103678(T)). The genome of Streptococcus azizii sp. nov. 12-5202(T) contains 2062 total genes with a size of 2.34 Mbp, and an average G+C content of 42.76 mol%. |
| Databáze: | OpenAIRE |
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