Cancer cell line microarray as a novel screening method for identification of radioresistance biomarkers in head and neck squamous cell carcinoma
| Autor: | Sami Ventelä, Karri Suvila, Jukka Westermarck, Reidar Grénman, Johannes Routila, Ilmo Leivo |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Microarray Cell Oct4 Biology Stem cell marker Radiation Tolerance Radiosensitivity 03 medical and health sciences 0302 clinical medicine Radioresistance Cell Line Tumor Genetics medicine Biomarkers Tumor Humans Gene Silencing Stemness RNA Small Interfering Head and neck cancer RC254-282 Aged Aged 80 and over Squamous Cell Carcinoma of Head and Neck Research Gene Expression Profiling Neoplasms. Tumors. Oncology. Including cancer and carcinogens Middle Aged medicine.disease Microarray Analysis Head and neck squamous-cell carcinoma Immunohistochemistry stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Cancer research Female RNA Interference |
| Zdroj: | BMC Cancer BMC Cancer, Vol 21, Iss 1, Pp 1-11 (2021) |
| ISSN: | 1471-2407 |
| Popis: | Background Currently, no clinically useful biomarkers for radioresistance are available in head and neck squamous cell carcinoma (HNSCC). This study assesses the usefulness of Cell Line Microarray (CMA) method to enhance immunohistochemical screening of potential immunohistochemical biomarkers for radioresistance in HNSCC cell lines. Methods Twenty-nine HNSCC cell lines were cultured, cell pellets formalin-fixed, paraffin-embedded, and arrayed. Radioresistance features of the cell lines were combined to immunohistochemical stains for p53, NDFIP1, EGFR, stem cell marker Oct4, and PP2A inhibitor CIP2A. Results Expression of p53, EGFR or CIP2A did not indicate intrinsic radioresistance in vitro. Stem cell marker Oct4 nuclear positivity and NDFIP1 nuclear positivity was correlated with increased intrinsic radioresistance. Conclusion The usefulness of CMA in analysis of HNSCC cell lines and discovery of biomarkers is demonstrated. CMA is very well adapted to both testing of antibodies in a large panel of cell lines as well as correlating staining results with other cell line characteristics. In addition, CMA-based antibody screening proved an efficient and relatively simple method to identify potential radioresistance biomarkers in HNSCC. |
| Databáze: | OpenAIRE |
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